Changes in the endopeptidase pattern of wheat leaves during senescence

Keist, Marcel; Feller, Urs (1985). Changes in the endopeptidase pattern of wheat leaves during senescence. Botanica Helvetica, 95(1), pp. 73-80. Birkhäuser 10.5169/seals-66502

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Peptide hydrolase activities present in young and senescing flag leaves of wheat (Triticum aestivum L.) were fractionated on DEAE-cellulose columns. Part of the endopeptidase activity was not bound to DEAE-cellulose at pH 7.5 (peak I) and had a broad pH Optimum (against acetylated casein) between 6 and 9. The other peaks (IIA and IIB) eluted in the NaCl gradient showed a pH optimum between 4 and 7. During senescence the endopeptidase pattern changed markedly in favour of peak I. Glutamine synthetase and nitrate reductase in extracts from young leaves were rapidly inactivated after addition of peak I-eluate, while peak Il-eluate was considerably less effective.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant nutrition (discontinued)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Feller, Urs

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0253-1453

Publisher:

Birkhäuser

Language:

German

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

07 Mar 2017 10:01

Last Modified:

18 May 2017 10:47

Publisher DOI:

10.5169/seals-66502

BORIS DOI:

10.7892/boris.91921

URI:

https://boris.unibe.ch/id/eprint/91921

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