Changing activities and different resistance to proteolytic activity of two forms of glutamine synthetase in wheat leaves during senescence

Streit, Luc; Feller, Urs (1983). Changing activities and different resistance to proteolytic activity of two forms of glutamine synthetase in wheat leaves during senescence. Physiologie végétale, 21(1), pp. 103-108. Gauthier-Villars

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Leaves of field-grown wheat (Triticum aestivum) were collected at different stages. Two forms of glutamine synthetase (GS I and GS II) were separated on diethylamino ethyl cellulose columns with a linear NaCl gradient. GS II was the predominant form in young leaves (85-92% of total activity). The activity of GS II decreased earlier than that of GS I during senescence, and therefore the ratio GS I/GS II changed in favour of GS I. Total glutamine synthetase was inactivated more rapidly in senescing leaves (high caseolytic activity) than in extracts of young leaves (low caseolytic activity). A more rapid inactivation of GS II than of GS I was found when extracts were pre-incubated at 30°C and then applied to a diethylamino ethyl cellulose column, suggesting that GS I was less susceptible to proteolysis than GS II. GS II is assumed to be located in the chloroplasts and GS I in the cytoplasm. Besides subcellular localization, the different stability may contribute to the changing ratio GS I/GS II.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant nutrition (discontinued)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Feller, Urs

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0031-9368

Publisher:

Gauthier-Villars

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

06 Jun 2017 13:30

Last Modified:

06 Jun 2017 13:30

Uncontrolled Keywords:

glutamine synthetase, isoforms, senescence, inactivation, wheat

BORIS DOI:

10.7892/boris.91930

URI:

https://boris.unibe.ch/id/eprint/91930

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