In Vitro Differentiation of Mouse Granulocytes.

Reinhart, Ramona; Wicki, Simone; Kaufmann, Thomas (2016). In Vitro Differentiation of Mouse Granulocytes. Methods in molecular biology, 1419, pp. 95-107. Humana Press 10.1007/978-1-4939-3581-9_8

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Granulocytes are central players of the immune system and, once activated, a tightly controlled balance between effector functions and cell removal by apoptosis guarantees maximal host benefit with least possible collateral damage to healthy tissue.Granulocytes are terminally differentiated cells that cannot be maintained in culture for prolonged times. Isolating primary granulocytes is inefficient and challenging when working with mice, and especially so for the lowly abundant eosinophil and basophil subtypes. Here we describe an in vitro protocol to massively expand mouse derived myeloid progenitors and to differentiate them "on demand" and in large numbers into mature neutrophils or basophils.

Item Type:

Journal Article (Further Contribution)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Pharmacology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Reinhart, Ramona; Wicki, Simone and Kaufmann, Thomas

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1064-3745

Publisher:

Humana Press

Language:

English

Submitter:

Jana Berger

Date Deposited:

29 Dec 2016 11:40

Last Modified:

15 Jun 2017 11:14

Publisher DOI:

10.1007/978-1-4939-3581-9_8

PubMed ID:

27108434

Uncontrolled Keywords:

Basophil; Differentiation; Granulocyte; Hoxb8; In vitro; Mouse; Neutrophil

BORIS DOI:

10.7892/boris.92202

URI:

https://boris.unibe.ch/id/eprint/92202

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