Novel genotype of Tritrichomonas foetus from cattle in Southern Africa

Casteriano, A; Molini, U; Kandjumbwa, K; Khaiseb, S; Frey, Caroline; Šlapeta, J (2016). Novel genotype of Tritrichomonas foetus from cattle in Southern Africa. Parasitology, 143(14), pp. 1954-1959. Cambridge University Press 10.1017/S003118201600158X

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Bovine trichomonosis caused by Tritrichomonas foetus is a significant reproductive disease of cattle. Preputial samples were collected using sheath washing technique in bulls in Namibia. Thirty-six trichomonad cultures were characterized using the TaqMan-probe commercial real-time polymerase chain reaction (PCR) diagnostic assay (VetMAX™-Gold Trich Detection Kit) and CYBR real-time PCR assay based on TFR3/4 primers. Diagnostic real-time PCRs and DNA sequencing of the internal transcribed region confirmed presence of T. foetus in 35 out of 36 samples. Multilocus genotyping using cysteine proteases (CP1, CP2, CP4, CP5, CP6, CP7, CP8, CP9) and malate dehydrogenase (MDH1) gene sequences demonstrate that the T. foetus in Namibia are genetically distinct from those characterized elsewhere. We report the discovery of a novel genotype of T. foetus in Namibian cattle, distinct from other T. foetus genotypes in Europe, South and North America and Australia. We suggest recognition of a 'Southern African' genotype of T. foetus. Identification of the new genotype of T. foetus demonstrates the need for wider global sampling to fully understand the diversity and origin of T. foetus causing disease in cattle or cats.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Frey, Caroline

Subjects:

600 Technology > 630 Agriculture

ISSN:

0031-1820

Publisher:

Cambridge University Press

Language:

English

Submitter:

Caroline Franziska Frey Marreros Canales

Date Deposited:

16 Feb 2017 10:54

Last Modified:

16 Feb 2017 10:54

Publisher DOI:

10.1017/S003118201600158X

PubMed ID:

27609311

BORIS DOI:

10.7892/boris.92214

URI:

https://boris.unibe.ch/id/eprint/92214

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