A dual PCR-based sequencing approach for the identification and discrimination of Echinococcus and Taenia taxa

Boubaker, Ghalia; Marinova, Irina; Gori, Francesca; Hizem, Amani; Müller, Norbert; Casulli, Adriano; Jerez Puebla, Luis Enrique; Babba, Hamouda; Gottstein, Bruno; Spiliotis, Markus (2016). A dual PCR-based sequencing approach for the identification and discrimination of Echinococcus and Taenia taxa. Molecular and cellular probes, 30(4), pp. 211-217. Elsevier 10.1016/j.mcp.2016.05.004

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Reliable and rapid molecular tools for the genetic identification and differentiation of Echinococcus species and/or genotypes are crucial for studying spatial and temporal transmission dynamics. Here, we describe a novel dual PCR targeting regions in the small (rrnS) and large (rrnL) subunits of mitochondrial ribosomal RNA (rRNA) genes, which enables (i) the specific identification of species and genotypes of Echinococcus (rrnS + L-PCR) and/or (ii) the identification of a range of taeniid cestodes, including different species of Echinococcus, Taenia and some others (17 species of diphyllidean helminths). This dual PCR approach was highly sensitive, with an analytical detection limit of 1 pg for genomic DNA of Echinococcus. Using concatenated sequence data derived from the two gene markers (1225 bp), we identified five unique and geographically informative single nucleotide polymorphisms (SNPs) that allowed genotypes (G1 and G3) of Echinococcus granulosus sensu stricto to be distinguished, and 25 SNPs that allowed differentiation within Echinococcus canadensis (G6/7/8/10). In conclusion, we propose that this dual PCR-based sequencing approach can be used for molecular epidemiological studies of Echinococcus and other taeniid cestodes.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Boubaker, Ghalia; Marinova, Irina; Hizem, Amani; Müller, Norbert; Gottstein, Bruno and Spiliotis, Markus

Subjects:

600 Technology > 630 Agriculture
600 Technology > 610 Medicine & health

ISSN:

0890-8508

Publisher:

Elsevier

Language:

English

Submitter:

Bruno Gottstein

Date Deposited:

13 Mar 2017 11:43

Last Modified:

16 Aug 2018 16:13

Publisher DOI:

10.1016/j.mcp.2016.05.004

PubMed ID:

27242008

Uncontrolled Keywords:

12S and 16S nuclear ribosomal RNA genes; Echinococcus spp.; PCR; Specific and genotypic identification; Taenia spp.

BORIS DOI:

10.7892/boris.92264

URI:

https://boris.unibe.ch/id/eprint/92264

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