Uptake of silica nanoparticles in the brain and effects on neuronal differentiation using different in vitro models

Ducray, Angélique; Stojiljkovic, Ana; Möller, Anja-Maria; Stoffel, Michael Hubert; Widmer, Hans Rudolf; Frenz, Martin; Mevissen, Meike (2017). Uptake of silica nanoparticles in the brain and effects on neuronal differentiation using different in vitro models. Nanomedicine : nanotechnology, biology, and medicine, 13(3), pp. 1195-1204. Elsevier 10.1016/j.nano.2016.11.001

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Nanomedicine offers a promising tool for therapies of brain diseases, but they may be associated with potential adverse effects. The aim of this study was to investigate the uptake of silica-nanoparticles engineered for laser-tissue soldering in the brain using SH-SY5Y cells, dissociated and organotypic slice cultures from rat hippocampus. Nanoparticles were predominantly taken up by microglial cells in the hippocampal cultures but nanoparticles were also found in differentiated SH-SY5Y cells. The uptake was time- and concentration-dependent in primary hippocampal cells. Transmission electron microscopy experiments demonstrated nanoparticle aggregates and single particles in the cytoplasm. Nanoparticles were found in the endoplasmic reticulum, but not in other cellular compartments. Nanoparticle exposure did not impair cell viability and neuroinflammation in primary hippocampal cultures at all times investigated. Neurite outgrowth was not significantly altered in SH-SY5Y cells, but the neuronal differentiation markers indicated a reduction in neuronal differentiation induction after nanoparticle exposure.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > NeuroCenter
08 Faculty of Science > Institute of Applied Physics
04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurosurgery
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Pharmacology and Toxicology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Anatomy
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Neurochirurgie
09 Interdisciplinary Units > Microscopy Imaging Center MIC

UniBE Contributor:

Ducray, Angélique; Stojiljkovic, Ana; Möller, Anja-Maria; Stoffel, Michael Hubert; Widmer, Hans Rudolf; Frenz, Martin and Mevissen, Meike

Subjects:

600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture
600 Technology > 620 Engineering
500 Science > 570 Life sciences; biology

ISSN:

1549-9642

Publisher:

Elsevier

Language:

English

Submitter:

Nicole Söll

Date Deposited:

09 Mar 2017 12:55

Last Modified:

11 Jan 2019 13:40

Publisher DOI:

10.1016/j.nano.2016.11.001

PubMed ID:

27871963

Uncontrolled Keywords:

Nanomedicine; Nanoparticle uptake; Neurodifferentiation; Neuroinflammation

BORIS DOI:

10.7892/boris.92424

URI:

https://boris.unibe.ch/id/eprint/92424

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