A pathogenic role for the integrin CD103 in experimental allergic airways disease.

Fear, Vanessa S; Lai, Siew Ping; Zosky, Graeme R; Perks, Kara L; Gorman, Shelley; Blank, Fabian; Von Garnier, Christophe; Stumbles, Philip A; Strickland, Deborah H (2016). A pathogenic role for the integrin CD103 in experimental allergic airways disease. Physiological reports, 4(21) The American Physiological Society 10.14814/phy2.13021

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The integrin CD103 is the αE chain of integrin αEβ7 that is important in the maintenance of intraepithelial lymphocytes and recruitment of T cells and dendritic cells (DC) to mucosal surfaces. The role of CD103 in intestinal immune homeostasis has been well described, however, its role in allergic airway inflammation is less well understood. In this study, we used an ovalbumin (OVA)-induced, CD103-knockout (KO) BALB/c mouse model of experimental allergic airways disease (EAAD) to investigate the role of CD103 in disease expression, CD4(+) T-cell activation and DC activation and function in airways and lymph nodes. We found reduced airways hyper-responsiveness and eosinophil recruitment to airways after aerosol challenge of CD103 KO compared to wild-type (WT) mice, although CD103 KO mice showed enhanced serum OVA-specific IgE levels. Following aerosol challenge, total numbers of effector and regulatory CD4(+) T-cell subsets were significantly increased in the airways of WT but not CD103 KO mice, as well as a lack of DC recruitment into the airways in the absence of CD103. While total airway DC numbers, and their in vivo allergen capture activity, were essentially normal in steady-state CD103 KO mice, migration of allergen-laden airway DC to draining lymph nodes was disrupted in the absence of CD103 at 24 h after aerosol challenge. These data support a role for CD103 in the pathogenesis of EAAD in BALB/c mice through local control of CD4(+) T cell and DC subset recruitment to, and migration from, the airway mucosa during induction of allergic inflammation.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Pneumologie (Erwachsene)
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology

UniBE Contributor:

Blank, Fabian and Von Garnier, Christophe

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2051-817X

Publisher:

The American Physiological Society

Language:

English

Submitter:

Rahel Holderegger

Date Deposited:

01 Feb 2017 10:21

Last Modified:

03 Feb 2017 18:22

Publisher DOI:

10.14814/phy2.13021

PubMed ID:

27905296

Uncontrolled Keywords:

Allergy; CD103; CD4 T cell; asthma; dendritic cell

BORIS DOI:

10.7892/boris.93088

URI:

https://boris.unibe.ch/id/eprint/93088

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