High-dose irradiation of bone chips preserves the in vitro activity of bone-conditioned medium.

Sawada, Kosaku; Miron, Richard John; Leiser, Dominic; Caballé Serrano, Jordi; Bosshardt, Dieter; Schaller, Benoît; Buser, Daniel; Gruber, Reinhard (2016). High-dose irradiation of bone chips preserves the in vitro activity of bone-conditioned medium. Journal of oral science, 58(3), pp. 325-331. Nihon University School of Dentistry 10.2334/josnusd.16-0042

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Extracorporeal irradiation sterilizes resected tumor bone used as autografts in reconstruction surgery. Therapeutic irradiation is a standard technique in head and neck cancer therapy that aims to preserve organ function. Bone irradiation has a complex, mostly inhibitory, effect on remodeling and regeneration, although the underlying mechanisms are still not fully understood. It remains unclear if extracorporeal irradiation affects the paracrine-like activity of the corresponding autografts. We recently reported that bone-conditioned medium from autogenous bone chips contains a number of factors that might affect cell activity. In the present study, we investigated the effects of extracorporeal irradiation of porcine cortical bone chips on the activity of the corresponding bone-conditioned medium. The effects of bone-conditioned medium on the expressions of transforming growth factor-beta (TGF-β) target genes in oral fibroblasts were assessed. Bone-conditioned medium from bone chips exposed to a total radiation dose up to 120 Gy did not affect expressions of TGF-β target genes, including adrenomedullin, BTB/POZ domain-containing protein 11, proteoglycan 4, NADPH oxidase 4, and interleukin 11, in oral fibroblasts. In conclusion, bone irradiation does not alter the capability of the corresponding bone-conditioned medium to provoke a robust fibroblastic cell response in vitro. (J Oral Sci 58, 325-331, 2016).

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Craniomaxillofacial Surgery
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Oral Surgery Research
04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Radiation Oncology
04 Faculty of Medicine > School of Dental Medicine > Department of Oral Surgery and Stomatology
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research

UniBE Contributor:

Sawada, Kosaku; Miron, Richard John; Leiser, Dominic; Caballé Serrano, Jordi; Bosshardt, Dieter; Schaller, Benoît; Buser, Daniel and Gruber, Reinhard

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1880-4926

Publisher:

Nihon University School of Dentistry

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

23 Mar 2017 14:28

Last Modified:

23 Mar 2017 14:28

Publisher DOI:

10.2334/josnusd.16-0042

PubMed ID:

27665970

BORIS DOI:

10.7892/boris.93137

URI:

https://boris.unibe.ch/id/eprint/93137

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