Seydoux, Emilie; Rodriguez-Lorenzo, Laura; Blom, Rebecca Agaatha Maria; Stumbles, Philip A; Petri-Fink, Alke; Rothen-Rutishauser, Barbara M; Blank, Fabian; Von Garnier, Christophe (2016). Pulmonary delivery of cationic gold nanoparticles boost antigen-specific CD4(+) T Cell Proliferation. Nanomedicine : nanotechnology, biology, and medicine, 12(7), pp. 1815-1826. Elsevier 10.1016/j.nano.2016.02.020
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To address how surface charge affects the fate of potential nanocarriers in the lung, gold nanoparticles (AuNPs) coated with polyvinyl alcohol containing either positively (NH2) or negatively (COOH) charged functional groups were intra-nasally instilled in mice, and their uptake by antigen presenting cell populations (APC) in broncho-alveolar lavage (BAL) fluid, trachea, and lung parenchyma, as well as trafficking to the lung draining lymph nodes (LDLNs) was assessed by flow cytometry. Furthermore, CD4(+) T cell proliferation in LDLNs was investigated following instillation. All APC subpopulations preferentially captured positively-charged AuNPs compared to their negatively-charged counterparts. Uptake of AuNPs up-regulated expression of co-stimulatory molecules on all APC populations. Furthermore, positively-charged AuNPs induced enhanced OVA-specific CD4(+) T cell stimulation in LDLNs compared to negatively-charged AuNPs, or polymer alone. Our findings demonstrate surface charge as a key parameter determining particle uptake by APC, and down-stream immune responses depend on the presence of particle core-bound polymer.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Pneumologie (Erwachsene) 04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology |
UniBE Contributor: |
Seydoux, Emilie, Blom, Rebecca Agaatha Maria, Blank, Fabian, von Garnier, Christophe |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1549-9642 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Rahel Holderegger |
Date Deposited: |
23 Mar 2017 16:29 |
Last Modified: |
02 Mar 2023 23:28 |
Publisher DOI: |
10.1016/j.nano.2016.02.020 |
PubMed ID: |
27013126 |
Additional Information: |
Emilie Seydoux has contributed to this article even after having left us by the end of 2015 when she terminated her education as doctoral candidate. |
Uncontrolled Keywords: |
Dendritic cell; Gold nanoparticles; Immune modulation; Macrophage; Respiratory tract |
BORIS DOI: |
10.7892/boris.93261 |
URI: |
https://boris.unibe.ch/id/eprint/93261 |
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