Does centrifugation matter? Centrifugal force and spinning time alter the plasma metabolome.

Lesche, Dorothea; Geyer, Roland; Lienhard, Daniel; Nakas, Christos T.; Diserens, Gaëlle; Vermathen, Peter; Leichtle, Alexander Benedikt (2016). Does centrifugation matter? Centrifugal force and spinning time alter the plasma metabolome. Metabolomics, 12(10), p. 159. Springer 10.1007/s11306-016-1109-3

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BACKGROUND Centrifugation is an indispensable procedure for plasma sample preparation, but applied conditions can vary between labs. AIM Determine whether routinely used plasma centrifugation protocols (1500×g 10 min; 3000×g 5 min) influence non-targeted metabolomic analyses. METHODS Nuclear magnetic resonance spectroscopy (NMR) and High Resolution Mass Spectrometry (HRMS) data were evaluated with sparse partial least squares discriminant analyses and compared with cell count measurements. RESULTS Besides significant differences in platelet count, we identified substantial alterations in NMR and HRMS data related to the different centrifugation protocols. CONCLUSION Already minor differences in plasma centrifugation can significantly influence metabolomic patterns and potentially bias metabolomics studies.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute of Clinical Chemistry
04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Nephrology and Hypertension
04 Faculty of Medicine > Department of Radiology, Neuroradiology and Nuclear Medicine (DRNN) > Institute of Diagnostic, Interventional and Paediatric Radiology > DCR Magnetic Resonance Spectroscopy and Methodology (AMSM)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Lesche, Dorothea; Geyer, Roland; Lienhard, Daniel; Nakas, Christos T.; Diserens, Gaëlle; Vermathen, Peter and Leichtle, Alexander Benedikt

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1573-3882

Publisher:

Springer

Language:

English

Submitter:

Christoph Hans Boesch

Date Deposited:

10 Feb 2017 08:40

Last Modified:

12 Feb 2017 02:12

Publisher DOI:

10.1007/s11306-016-1109-3

PubMed ID:

27729833

Uncontrolled Keywords:

Centrifugation; Metabolome; Plasma; Preanalytics; Relative centrifugal force; Spinning time

BORIS DOI:

10.7892/boris.93297

URI:

https://boris.unibe.ch/id/eprint/93297

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