The non-canonical mitochondrial inner membrane protein translocase of trypanosomatids contains two essential rhomboid-like proteins

Schneider, André (4 September 2016). The non-canonical mitochondrial inner membrane protein translocase of trypanosomatids contains two essential rhomboid-like proteins (Unpublished). In: BSP Trypanosomiasis & Leishmaniasis Seminar 2016. Ceske Budejovice, Czech Republic. 04.-07.09.2016.

Mitochondrial protein import is essential for all eukaryotes. Here we show that the early diverging eukaryote Trypanosoma brucei has a non-canonical inner membrane protein translocase (TIM) which unlike in other systems appears to mediate import of both presequence-containing and mitochondrial carrier proteins. We have analyzed the composition of the trypanosomal TIM complex. Besides TbTim17, which is the single member of the Tim17/22/23 family in trypanosomes, it contains nine subunits that are co-purified in reciprocal immunoprecipitations and with an import substrate that is trapped in the translocation channel. Two of the newly discovered TIM subunits are rhomboid-like proteins, which are essential for growth and mitochondrial protein import. Rhomboid-like proteins were proposed to form the protein translocation pore of the ER-associated degradation system (ERAD). No further candidates for the translocation channel are found among the subunits of the trypanosomal TIM complex, suggesting that the rhomboid-like proteins may contribute to pore formation.

Item Type:

Conference or Workshop Item (Speech)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Schneider, André

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

Language:

English

Submitter:

Christina Schüpbach

Date Deposited:

26 Jan 2017 11:33

Last Modified:

26 Jan 2017 11:33

URI:

https://boris.unibe.ch/id/eprint/93319

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