Effect of recombinant PDGF-BB on bone formation in the presence of β-tricalcium phosphate and bovine bone mineral matrix: a pilot study in rat calvarial defects.

Luvizuto, Eloá R; Tangl, Stefan; Dobsak, Toni; Reich, Karoline; Gruber, Reinhard; Sonoda, Celso K; Okamoto, Roberta (2016). Effect of recombinant PDGF-BB on bone formation in the presence of β-tricalcium phosphate and bovine bone mineral matrix: a pilot study in rat calvarial defects. BMC Oral Health, 16(1)(52), p. 52. BioMed Central 10.1186/s12903-016-0210-3

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BACKGROUND Supplementation of bone substitutes with recombinant platelet-derived growth factor-BB (PDGF-BB) can enhance bone regeneration. The aim of the study was to evaluate the effect of PDGF-BB on bone formation in the presence of β-tricalcium phosphate and bovine bone mineral matrix in a rat calvaria defect model. METHODS The authors examined 5 mm rat calvarial defects treated with β-tricalcium phosphate (TCP) or demineralized bovine bone mineral (DBBM) with and without 0.3 mg/ml recombinant PDGF-BB. Calvaria defects were randomly divided into the following treatment groups (n = 5); TCP; TCP plus PDGF-BB; DBBM; DBBM plus PDGF-BB; and untreated empty control. After 45 days, bone formation was evaluated by histomorphometry and fluorescence microscopy. RESULTS The authors report that the area of newly formed bone was similar between the empty controls and the two bone substitutes, TCP and DBBM. Supplementation of TCP and DBBM with PDGF-BB had no significant impact on bone formation. Fluorochrome staining revealed no visible changes in the pattern of bone formation in defects filled with TCP and DBBM, irrespective of PDGF-BB. Furthermore, supplementation with PDGF-BB did not influence biomaterial degradation. CONCLUSIONS The authors concluded that PDGF-BB had no impact on bone formation and degradation of bone substitutes in the respective rodent models. Thus, possible beneficial effects of PDGF-BB may require other model situations.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Restorative Dentistry, Research
04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry

UniBE Contributor:

Gruber, Reinhard

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1472-6831

Publisher:

BioMed Central

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

26 Jan 2017 16:41

Last Modified:

29 Jan 2017 02:13

Publisher DOI:

10.1186/s12903-016-0210-3

PubMed ID:

27145819

Uncontrolled Keywords:

Bone Augmentation, Bone formation, Bone Regeneration, Bone substitutes, Demineralized bovine bone Mineral, Graft consolidation, Histomorphometry, Platelet-derived growth factor-BB, Rat calvaria defects β-tricalcium phosphate

BORIS DOI:

10.7892/boris.93915

URI:

https://boris.unibe.ch/id/eprint/93915

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