Mesenchymal Remodeling during Palatal Shelf Elevation Revealed by Extracellular Matrix and F-Actin Expression Patterns.

Chiquet, Matthias; Blumer, Susan; Angelini, Manuela; Mitsiadis, Thimios A; Katsaros, Christos (2016). Mesenchymal Remodeling during Palatal Shelf Elevation Revealed by Extracellular Matrix and F-Actin Expression Patterns. Frontiers in physiology, 7(392), p. 392. Frontiers Research Foundation 10.3389/fphys.2016.00392

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During formation of the secondary palate in mammalian embryos, two vertically oriented palatal shelves rapidly elevate into a horizontal position above the tongue, meet at the midline, and fuse to form a single entity. Previous observations suggested that elevation occurs by a simple 90° rotation of the palatal shelves. More recent findings showed that the presumptive midline epithelial cells are not located at the tips of palatal shelves before elevation, but mostly toward their medial/lingual part. This implied extensive tissue remodeling during shelf elevation. Nevertheless, it is still not known how the shelf mesenchyme reorganizes during this process, and what mechanism drives it. To address this question, we mapped the distinct and restricted expression domains of certain extracellular matrix components within the developing palatal shelves. This procedure allowed to monitor movements of entire mesenchymal regions relative to each other during shelf elevation. Consistent with previous notions, our results confirm a flipping movement of the palatal shelves anteriorly, whereas extensive mesenchymal reorganization is observed more posteriorly. There, the entire lingual portion of the vertical shelves moves close to the midline after elevation, whereas the mesenchyme at the original tip of the shelves ends up ventrolaterally. Moreover, we observed that the mesenchymal cells of elevating palatal shelves substantially align their actin cytoskeleton, their extracellular matrix, and their nuclei in a ventral to medial direction. This indicates that, like in other morphogenetic processes, actin-dependent cell contractility is a major driving force for mesenchymal tissue remodeling during palatogenesis.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Orthodontics
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Orthodontic Research

UniBE Contributor:

Chiquet, Matthias; Angelini, Manuela and Katsaros, Christos

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1664-042X

Publisher:

Frontiers Research Foundation

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

05 May 2017 15:16

Last Modified:

07 May 2017 02:23

Publisher DOI:

10.3389/fphys.2016.00392

PubMed ID:

27656150

Uncontrolled Keywords:

actin, extracellular Matrix, mouse Embryo, palatal shelf Elevation, palate Morphogenesis, tissue remodeling

BORIS DOI:

10.7892/boris.93949

URI:

https://boris.unibe.ch/id/eprint/93949

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