Histochemical changes of occlusal surface enamel of permanent teeth, where dental caries is questionable vs sound enamel surfaces.

Michalaki, M; Oulis, C J; Pandis, Nikolaos; Eliades, G (2016). Histochemical changes of occlusal surface enamel of permanent teeth, where dental caries is questionable vs sound enamel surfaces. European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry, 17(6), pp. 445-454. Springer 10.1007/s40368-016-0252-x

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AIM This in vitro study was to classify questionable for caries occlusal surfaces (QCOS) of permanent teeth according to ICDAS codes 1, 2, and 3 and to compare them in terms of enamel mineral composition with the areas of sound tissue of the same tooth. METHODS Partially impacted human molars (60) extracted for therapeutic reasons with QCOS were used in the study, photographed via a polarised light microscope and classified according to the ICDAS II (into codes 1, 2, or 3). The crowns were embedded in clear self-cured acrylic resin and longitudinally sectioned at the levels of the characterised lesions and studied by SEM/EDX, to assess enamel mineral composition of the QCOS. Univariate and multivariate random effect regressions were used for Ca (wt%), P (wt%), and Ca/P (wt%). RESULTS The EDX analysis indicated changes in the Ca and P contents that were more prominent in ICDAS-II code 3 lesions compared to codes 1 and 2 lesions. In these lesions, Ca (wt%) and P (wt%) concentrations were significantly decreased (p = 0.01) in comparison with sound areas. Ca and P (wt%) contents were significantly lower (p = 0.02 and p = 0.01 respectively) for code 3 areas in comparison with codes 1 and 2 areas. Significantly higher (p = 0.01) Ca (wt%) and P (wt%) contents were found on sound areas compared to the lesion areas. CONCLUSIONS The enamel of occlusal surfaces of permanent teeth with ICDAS 1, 2, and 3 lesions was found to have different Ca/P compositions, necessitating further investigation on whether these altered surfaces might behave differently on etching preparation before fissure sealant placement, compared to sound surfaces.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Orthodontics

UniBE Contributor:

Pandis, Nikolaos

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1818-6300

Publisher:

Springer

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

19 Apr 2017 11:02

Last Modified:

18 Apr 2018 14:59

Publisher DOI:

10.1007/s40368-016-0252-x

Related URLs:

PubMed ID:

27866328

Uncontrolled Keywords:

Altered enamel composition; ICDAS-II codes 1 and 2; Incipient lesions; Questionable for caries occlusal surfaces

BORIS DOI:

10.7892/boris.94037

URI:

https://boris.unibe.ch/id/eprint/94037

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