Impedance aggregometric analysis of platelet function of apheresis platelet concentrates as a function of storage time.

Glas, Michael; Bauer, Janine Viola; Eichler, Hermann; Volk, Thomas (2016). Impedance aggregometric analysis of platelet function of apheresis platelet concentrates as a function of storage time. Scandinavian journal of clinical and laboratory investigation, 76(8), pp. 664-670. Taylor & Francis 10.1080/00365513.2016.1238505

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Multiple electrode (impedance) aggregometry (MEA) allows reliable monitoring of platelet function in whole blood. The aims of the present study were to implement MEA for analyzing aggregation in platelet concentrates and to correlate results with storage time and blood gas analysis (BGA). We investigated the influence of platelet counts, calcium concentrations and agonists on platelet aggregation. Samples of apheresis concentrates up to an age of 12 days were investigated by MEA and BGA. For ASPI- and TRAPtest MEA was reproducible for a platelet count of 400 per 10(-9 )L and a calcium concentration of 5 mmol L(-1). Platelets at the age of 2-4 days yielded steady aggregation. Platelet concentrates exceeding the storage time for transfusion showed steady aggregation up to 10 days, but a significant decline on day 12. Weak correlation was found regarding pCO2 and MEA as well as regarding glucose concentration and MEA. Our results indicate that MEA is applicable for evaluation of aggregation in stored apheresis concentrates. Prolonged storage seems not to be prejudicial regarding platelet aggregation. Platelet concentrates showed acceptable BGA throughout storage time. Further studies are required to evaluate the application of MEA for quality controls in platelet concentrates.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Intensive Care, Emergency Medicine and Anaesthesiology (DINA) > Clinic of Intensive Care

UniBE Contributor:

Glas, Michael

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0036-5513

Publisher:

Taylor & Francis

Language:

English

Submitter:

Mirella Aeberhard

Date Deposited:

26 Apr 2017 09:27

Last Modified:

26 Apr 2017 09:27

Publisher DOI:

10.1080/00365513.2016.1238505

PubMed ID:

27701904

Uncontrolled Keywords:

Blood preservation; electric impedance; platelet aggregation; platelet function tests; plateletpheresis

URI:

https://boris.unibe.ch/id/eprint/94182

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