Molecular characterization of Echinococcus granulosus isolates from Bulgarian human cystic echinococcosis patients

Marinova, Irina; Spiliotis, Markus; Wang, Junhua; Muhtarov, Marin; Chaligiannis, Ilias; Sotiraki, Smaro; Rainova, Iskra; Gottstein, Bruno; Boubaker, Ghalia (2017). Molecular characterization of Echinococcus granulosus isolates from Bulgarian human cystic echinococcosis patients. Parasitology research, 116(3), pp. 1043-1054. Springer 10.1007/s00436-017-5386-1

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Although cystic echinococcosis (CE) is highly endemic in Bulgaria, there is still scarce information about species and/or genotypes of the Echinococcus granulosus complex that infect humans. Our study tackled the genetic diversity of E. granulosus complex in a cohort of 30 Bulgarian CE patients. Ten animal E. granulosus isolates from neighboring Greece were additionally included. Specimens were comparatively analyzed for partial sequences of five mitochondrial (mt) (cox I, nad I, rrnS, rrnL, and atp6) and three nuclear (nc) genes (act II, hbx 2, and ef-1α) using a PCR-sequencing approach. All 30 Bulgarian isolates were identified as E. granulosus sensu stricto (s.s.) and were showing identical sequences for each of the three examined partial nc gene markers. Based upon concatenated sequences from partial mtDNA markers, we detected 10 haplotypes: 6 haplotypes (H1-H6) clustering with E. granulosus s.s. (G1) and 4 haplotypes (H9-H13) grouping with E. granulosus s.s. (G3), with H1 and H10 being the most frequent in Bulgarian patients. The haplotypes H1, H4, and H11 were also present in Greek hydatid cyst samples of animal origin. In conclusion, E. granulosus s.s. (G1 and G3 genotypes) is the only causative agent found so far to cause human CE in Bulgaria. However, further studies including larger sample sizes and other additional geographic regions in Bulgaria will have to be performed to confirm our results.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Marinova, Irina; Spiliotis, Markus; Wang, Junhua; Gottstein, Bruno and Boubaker, Ghalia

Subjects:

600 Technology > 630 Agriculture

ISSN:

0932-0113

Publisher:

Springer

Language:

English

Submitter:

Susanne Portner

Date Deposited:

06 Jul 2017 15:41

Last Modified:

06 Jul 2017 15:41

Publisher DOI:

10.1007/s00436-017-5386-1

PubMed ID:

28110440

Uncontrolled Keywords:

Echinococcus spp.; Genotyping; Mitochondrial markers; Nuclear markers; PCR; Speciation

BORIS DOI:

10.7892/boris.94401

URI:

https://boris.unibe.ch/id/eprint/94401

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