Progestin suppressed inflammation and cell viability of tumor necrosis factor-α-stimulated endometriotic stromal cells.

Grandi, Giovanni; Mueller, Michael; Bersinger, Nick A.; Papadia, Andrea; Nirgianakis, Konstantinos; Cagnacci, Angelo; Mc Kinnon, Brett (2016). Progestin suppressed inflammation and cell viability of tumor necrosis factor-α-stimulated endometriotic stromal cells. American journal of reproductive immunology, 76(4), pp. 292-298. Wiley 10.1111/aji.12552

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PROBLEM Endometriosis is an estrogen-dependent inflammatory disease. Progestins are a first-line treatment for endometriosis via activation of pituitary progesterone receptors and suppression of systemic estrogen: a less than optimal treatment. Increasing evidence is beginning to show that progestins may also influence local endometriotic cells, which may contribute to their clinical efficacy. METHOD OF STUDY Endometrial stromal cells (ESC) isolated from women with endometriosis were cultured with TNF-α to simulate an inflammatory environment. ESC were treated with the progestins, medroxyprogesterone acetate (MPA), norethisterone acetate (NETA), or dienogest (DNG) and cytokine mRNA production, protein secretion, and cell viability measured. RESULTS DNG, NETA, and MPA suppressed the secretion of interleukin (IL)-6, IL-8, and monocyte chemotactic protein (MCP)-1 from ESC. DNG and NETA only reduced the TNF-α-stimulated mRNA production. All three progestins suppressed TNF-α-stimulated ESC proliferation. CONCLUSION Progestins may influence endometriotic stromal cells altering the inflammatory microenvironment and their clinical efficacy.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Endometriose und gynäkologische Onkologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Endometriose und gynäkologische Onkologie

UniBE Contributor:

Grandi, Giovanni; Mueller, Michael; Bersinger, Nick A.; Papadia, Andrea; Nirgianakis, Konstantinos and Mc Kinnon, Brett

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1046-7408

Publisher:

Wiley

Language:

English

Submitter:

Monika Zehr

Date Deposited:

28 Apr 2017 13:54

Last Modified:

28 Apr 2017 13:54

Publisher DOI:

10.1111/aji.12552

PubMed ID:

27515307

Uncontrolled Keywords:

dienogest; endometriosis; inflammation; medroxyprogesterone acetate; norethisterone acetate

BORIS DOI:

10.7892/boris.94513

URI:

https://boris.unibe.ch/id/eprint/94513

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