Inflammation influences steroid hormone receptors targeted by progestins in endometrial stromal cells from women with endometriosis.

Grandi, Giovanni; Mueller, Michael; Papadia, Andrea; Kocbek, Vida; Bersinger, Nick A.; Petraglia, Felice; Cagnacci, Angelo; Mc Kinnon, Brett (2016). Inflammation influences steroid hormone receptors targeted by progestins in endometrial stromal cells from women with endometriosis. Journal of reproductive immunology, 117, pp. 30-38. Elsevier 10.1016/j.jri.2016.06.004

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Endometriosis is an estrogen-dependent disease characterised by the growth of endometrial epithelial and stromal cells outside the uterus creating a chronic inflammatory environment that further contributes to disease progression. The first choice treatment for endometriosis is currently progestin mediated hormone modulation. In addition to their progestogenic activity however, progestins also have the potential to bind to other nuclear receptors influencing their local activity on endometriotic cells. This local activity will be dependent on the steroid hormone receptor expression that occurs in endometrial cells in a chronic inflammatory environment. We therefore aimed to quantify receptors targeted by progestins in endometrial stromal cells after exposure to inflammation. Using primary endometrial stromal cells isolated from women with endometriosis we examined the mRNA and protein expression of the progesterone receptors A and B, membrane progesterone receptors 1 and 2, androgen receptors, mineralocorticoid receptors and glucocorticoid receptors after exposure to the inflammatory cytokines tumor necrosis factor α (TNFα) and interleukin 1β (IL-1β). The results indicate that both cytokines reduced the expression of progesterone receptors and increased the expression of the glucocorticoid receptors in the endometrial stromal cells. The change in expression of progestin targets in endometrial stromal cells in an inflammatory environment could contribute to the progesterone resistance observed in endometriotic cells and ultimately influence the design of hormonal therapies aimed at treating this disease.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Endometriose und gynäkologische Onkologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Endometriose und gynäkologische Onkologie

UniBE Contributor:

Grandi, Giovanni; Mueller, Michael; Papadia, Andrea; Kocbek, Vida; Bersinger, Nick A. and Mc Kinnon, Brett

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1872-7603

Publisher:

Elsevier

Language:

English

Submitter:

Monika Zehr

Date Deposited:

28 Apr 2017 14:57

Last Modified:

28 Apr 2017 14:57

Publisher DOI:

10.1016/j.jri.2016.06.004

PubMed ID:

27371899

Uncontrolled Keywords:

Androgen receptor; Endometriosis; Glucocorticoid receptor; Inflammation; Membrane progesterone receptor; Mineralocorticoid receptor; Progesterone receptor; Progestin

BORIS DOI:

10.7892/boris.94515

URI:

https://boris.unibe.ch/id/eprint/94515

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