Distribution of Giardia duodenalis Assemblages by PCR-RFLP of β-Giardin Gene in Cuban Children

Puebla, Luis; Núñez, Fidel; Rivero, Lázara; Silva, Isabel; Valdés, Lucia; Millán, Iraís; Müller, Norbert (2016). Distribution of Giardia duodenalis Assemblages by PCR-RFLP of β-Giardin Gene in Cuban Children. Journal of pediatric infectious diseases, 11(01), 006-012. Georg Thieme Verlag 10.1055/s-0036-1586212

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Giardia duodenalis is one of the most prevalent intestinal parasites worldwide that infect humans, especially children. This study was aimed to determine the prevalence of assemblages of Giardia among a group of children. A descriptive cross-sectional study was performed in the Pediatric Academic Hospital “William Soler” from January 2014 to September 2014 in 286 children as part of a surveillance program of intestinal parasitic infections in scholar children. Out of 286 samples examined, 27 were positive for G. duodenalis for an infection rate of 9.34%. All Giardia positive samples were successfully
amplified by a polymerase chain reaction (PCR) assay targeting the β-giardin gene. Restriction fragment length polymorphism analysis of these PCR products revealed that children infected with assemblage B were more prevalent (19/27; 70.4%) than those who harbored assemblage A (8/27; 29.6%) of this parasite. Abdominal pain was statistically associated with the infecting assemblage B of Giardia. The sequence of PCR products of β-giardin gene from symptomatic children infected with assemblage A revealed a distribution of four subassemblages AII divided in two subtypes A3 and two A2. Cases infected with subassemblage AII-A3 had a more abrupt onset of symptomatology.
These findings highlighted the use of novel molecular tools for a better
discrimination of assemblages at the subassemblages and genotypes levels are needed to verify possible correlations between Giardia genotypes and symptomatology of giardiasis.

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