Adhesion of Porphyromonas gingivalis and Tannerella forsythia to dentin and titanium with sandblasted and acid etched surface coated with serum and serum proteins - An in vitro study.

Eick, Sigrun; Kindblom, Christian; Mizgalska, Danuta; Magdon, Anna; Jurczyk, Karolina; Sculean, Anton; Stavropoulos, Andreas (2017). Adhesion of Porphyromonas gingivalis and Tannerella forsythia to dentin and titanium with sandblasted and acid etched surface coated with serum and serum proteins - An in vitro study. Archives of oral biology, 75, pp. 81-88. Elsevier Science 10.1016/j.archoralbio.2016.11.001

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OBJECTIVE To evaluate the adhesion of selected bacterial strains incl. expression of important virulence factors at dentin and titanium SLA surfaces coated with layers of serum proteins. METHODS Dentin- and moderately rough SLA titanium-discs were coated overnight with human serum, or IgG, or human serum albumin (HSA). Thereafter, Porphyromonas gingivalis, Tannerella forsythia, or a six-species mixture were added for 4h and 24h. The number of adhered bacteria (colony forming units; CFU) was determined. Arg-gingipain activity of P. gingivalis and mRNA expressions of P. gingivalis and T. forsythia proteases and T. forsythia protease inhibitor were measured. RESULTS Coating specimens never resulted in differences exceeding 1.1 log10 CFU, comparing to controls, irrespective the substrate. Counts of T. forsythia were statistically significantly higher at titanium than dentin, the difference was up to 3.7 log10 CFU after 24h (p=0.002). No statistically significant variation regarding adhesion of the mixed culture was detected between surfaces or among coatings. Arg-gingipain activity of P. gingivalis was associated with log10 CFU but not with the surface or the coating. Titanium negatively influenced mRNA expression of T. forsythia protease inhibitor at 24h (p=0.026 uncoated, p=0.009 with serum). CONCLUSIONS The present findings indicate that: a) single bacterial species (T. forsythia) can adhere more readily to titanium SLA than to dentin, b) low expression of T. forsythia protease inhibitor may influence the virulence of the species on titanium SLA surfaces in comparison with teeth, and c) surface properties (e.g. material and/or protein layers) do not appear to significantly influence multi-species adhesion.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine

UniBE Contributor:

Eick, Sigrun; Magdon, Anna; Jurczyk, Karolina and Sculean, Anton

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0003-9969

Publisher:

Elsevier Science

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

10 May 2017 10:50

Last Modified:

01 Apr 2018 02:30

Publisher DOI:

10.1016/j.archoralbio.2016.11.001

PubMed ID:

27825675

Uncontrolled Keywords:

Bacterial proteases, Implant surface, Porphyromonas gingivalis, Proteinaceous layer, Tannerella forsythia

BORIS DOI:

10.7892/boris.94800

URI:

https://boris.unibe.ch/id/eprint/94800

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