Dentifrices for children differentially affect cell viability in vitro.

Cvikl, Barbara; Lussi, Adrian; Moritz, Andreas; Gruber, Reinhard (2017). Dentifrices for children differentially affect cell viability in vitro. Clinical oral investigations, 21(1), pp. 453-461. Springer 10.1007/s00784-016-1813-4

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OBJECTIVES Child dentifrices vary in their composition, with possible differential impacts on cells in the oral soft tissue. While cytotoxicity studies have been performed on adult dentifrices, no respective studies have thus far been reported on child dentifrices. MATERIAL AND METHODS Seventeen commercial dentifrices for children up to 12 years of age were evaluated with respect to their in vitro cytotoxicity on gingival fibroblasts, oral squamous cell carcinoma HSC-2 cells, and L929 mouse fibroblasts. Proliferation was analyzed and live-dead staining was performed. RESULTS Ten child dentifrices greatly reduced cell viability with LC50 values below 5 %. Four dentifrices showed a moderate cytotoxicity with LC50 values between 5 and 20 %. Three child dentifrices showed almost no cytotoxicity with LC50 values above 95 %. The results of the assays for proliferation and live-dead staining supported these findings. CONCLUSIONS The different composition of the child dentifrices translated into a broad spectrum of in vitro cytotoxicity on cells of the oral cavity. CLINICAL RELEVANCE The in vitro data provide the scientific foundation for further in vivo research testing the clinical relevance of the present findings.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry

UniBE Contributor:

Cvikl, Barbara; Lussi, Adrian and Gruber, Reinhard

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1432-6981

Publisher:

Springer

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

10 May 2017 13:26

Last Modified:

10 May 2017 13:26

Publisher DOI:

10.1007/s00784-016-1813-4

PubMed ID:

27052616

Uncontrolled Keywords:

Children, Cytotoxicity, Dentifrices, In vitro Toothpaste

BORIS DOI:

10.7892/boris.94810

URI:

https://boris.unibe.ch/id/eprint/94810

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