Vascularization after treatment of gingival recession defects with platelet-rich fibrin or connective tissue graft.

Eren, Gülnihal; Kantarcı, Alpdoğan; Sculean, Anton; Atilla, Gül (2016). Vascularization after treatment of gingival recession defects with platelet-rich fibrin or connective tissue graft. Clinical oral investigations, 20(8), pp. 2045-2053. Springer 10.1007/s00784-015-1697-8

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OBJECTIVE The aim of this study was to evaluate histologically the following treatment of bilateral localized gingival recessions with coronally advanced flap (CAF) combined with platelet-rich fibrin (PRF) or subepithelial connective tissue graft (SCTG). MATERIALS AND METHODS Tissue samples were harvested from 14 subjects either 1 or 6 months after the surgeries. The 2-mm punch biopsies were obtained from the mid-portion of the grafted sites. Neutral buffered formalin fixed, paraffin-embedded 5-μm thick tissue sections were stained with hematoxylin eosin and Masson's trichrome in order to analyze the collagen framework, epithelium thickness and rete-peg length. Multiple sequential sections were cut from paraffin-embedded blocks of tissue and immunohistochemically prepared for detection of vascular endothelial growth factor, CD31 and CD34, for the assessment of vascularization. RESULTS Rete peg formation was significantly increased in the sites treated with PRF compared to the SCTG group after 6 months (p < 0.05). On the contrary, the number of vessels was increased in the SCTG group compared to the PRF group after 6 months (p < 0.05). No statistically significant differences were observed in the collagen density. Staining intensity of CD31 increased in submucosal area of PRF group than SCTG group after 1 month. Higher staining intensity of CD34 was observed in the submucosal area of PRF group compared with SCTG group after 6 months. CONCLUSIONS The results of the present study suggest that in histological evaluation because of its biological compounds, PRF results earlier vessel formation and tissue maturation compared to connective tissue graft. CLINICAL RELEVANCE PRF regulated the vascular response associated with an earlier wound healing.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine

UniBE Contributor:

Sculean, Anton

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1432-6981

Publisher:

Springer

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

24 Apr 2017 15:51

Last Modified:

24 Apr 2017 15:51

Publisher DOI:

10.1007/s00784-015-1697-8

PubMed ID:

26696115

Uncontrolled Keywords:

Gingival recession, Histology, Platelet-rich Fibrin, Subepithelial connective tissue graft, Wound healing

BORIS DOI:

10.7892/boris.94856

URI:

https://boris.unibe.ch/id/eprint/94856

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