Cellular responses after exposure of lung cell cultures to secondary organic aerosol particles

Gaschen, Annina; Lang, Doris; Kalberer, Markus; Savi, Melanie; Geiser, Thomas; Gazdhar, Amiq; Lehr, Claus-Michael; Bur, Michael; Dommen, Josef; Baltensperger, Urs; Geiser, Marianne (2010). Cellular responses after exposure of lung cell cultures to secondary organic aerosol particles. Environmental science & technology, 44(4), pp. 1424-30. Washington, D.C.: ACS Publications 10.1021/es902261m

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The scope of this work was to examine in vitro responses of lung cells to secondary organic aerosol (SOA) particles, under realistic ambient air and physiological conditions occurring when particles are inhaled by mammals, using a novel particle deposition chamber. The cell cultures included cell types that are representative for the inner surface of airways and alveoli and are the target cells for inhaled particles. The results demonstrate that an exposure to SOA at ambient-air concentrations of about 10(4) particles/cm(3) for 2 h leads to only moderate cellular responses. There is evidence for (i) cell type specific effects and for (ii) different effects of SOA originating from anthropogenic and biogenic precursors, i.e. 1,3,5-trimethylbenzene (TMB) and alpha-pinene, respectively. There was no indication for cytotoxic effects but for subtle changes in cellular functions that are essential for lung homeostasis. Decreased phagocytic activity was found in human macrophages exposed to SOA from alpha-pinene. Alveolar epithelial wound repair was affected by TMB-SOA exposure, mainly because of altered cell spreading and migration at the edge of the wound. In addition, cellular responses were found to correlate with particle number concentration, as interleukin-8 production was increased in pig explants exposed to TMB-SOA with high particle numbers.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology 04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Cell Biology 04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
UniBE Contributor:	Geiser, Thomas (A), Gazdhar, Amiq, Geiser, Marianne
ISSN:	0013-936X
Publisher:	ACS Publications
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:09
Last Modified:	29 Mar 2023 23:31
Publisher DOI:	10.1021/es902261m
PubMed ID:	20092303
Web of Science ID:	000274347800043
URI:	https://boris.unibe.ch/id/eprint/949 (FactScience: 201546)