Cellular responses after exposure of lung cell cultures to secondary organic aerosol particles

Gaschen, Annina; Lang, Doris; Kalberer, Markus; Savi, Melanie; Geiser, Thomas; Gazdhar, Amiq; Lehr, Claus-Michael; Bur, Michael; Dommen, Josef; Baltensperger, Urs; Geiser, Marianne (2010). Cellular responses after exposure of lung cell cultures to secondary organic aerosol particles. Environmental science & technology, 44(4), pp. 1424-30. Washington, D.C.: ACS Publications 10.1021/es902261m

Full text not available from this repository. (Request a copy)

The scope of this work was to examine in vitro responses of lung cells to secondary organic aerosol (SOA) particles, under realistic ambient air and physiological conditions occurring when particles are inhaled by mammals, using a novel particle deposition chamber. The cell cultures included cell types that are representative for the inner surface of airways and alveoli and are the target cells for inhaled particles. The results demonstrate that an exposure to SOA at ambient-air concentrations of about 10(4) particles/cm(3) for 2 h leads to only moderate cellular responses. There is evidence for (i) cell type specific effects and for (ii) different effects of SOA originating from anthropogenic and biogenic precursors, i.e. 1,3,5-trimethylbenzene (TMB) and alpha-pinene, respectively. There was no indication for cytotoxic effects but for subtle changes in cellular functions that are essential for lung homeostasis. Decreased phagocytic activity was found in human macrophages exposed to SOA from alpha-pinene. Alveolar epithelial wound repair was affected by TMB-SOA exposure, mainly because of altered cell spreading and migration at the edge of the wound. In addition, cellular responses were found to correlate with particle number concentration, as interleukin-8 production was increased in pig explants exposed to TMB-SOA with high particle numbers.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Cell Biology
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Geiser, Thomas; Gazdhar, Amiq Ur Rahman and Geiser, Marianne

ISSN:

0013-936X

Publisher:

ACS Publications

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:09

Last Modified:

18 Jan 2017 10:29

Publisher DOI:

10.1021/es902261m

PubMed ID:

20092303

Web of Science ID:

000274347800043

URI:

https://boris.unibe.ch/id/eprint/949 (FactScience: 201546)

Actions (login required)

Edit item Edit item
Provide Feedback