Distinct T helper cell dependence of memory B-cell proliferation versus plasma cell differentiation

Zabel, Franziska; Fettelschoss, Antonia; Vogel, Monique; Johansen, Pål; Kündig, Thomas M; Bachmann, Martin (2017). Distinct T helper cell dependence of memory B-cell proliferation versus plasma cell differentiation. Immunology, 150(3), pp. 329-342. Wiley-Blackwell 10.1111/imm.12688

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Several memory B-cell subclasses with distinct functions have been described, of which the most effective is the class-switched (CS) memory B-cell population. We have previously shown, using virus-like particles (VLPs), that the proliferative potential of these CS memory B cells is limited and they fail to re-enter germinal centres (GCs). However, VLP-specific memory B cells quickly differentiated into secondary plasma cells (PCs) with the virtue of elevated antibody production compared with primary PCs. Whereas the induction of VLP(+) memory B cells was strongly dependent on T helper cells, we were wondering whether re-stimulation of VLP(+) memory B cells and their differentiation into secondary PCs would also require T helper cells. Global absence of T helper cells led to strongly impaired memory B cell proliferation and PC differentiation. In contrast, lack of interleukin-21 receptor-dependent follicular T helper cells or CD40 ligand signalling strongly affected proliferation of memory B cells, but differentiation into mature secondary PCs exhibiting increased antibody production was essentially normal. This contrasts with primary B-cell responses, where a strong dependence on CD40 ligand but limited importance of interleukin-21 receptor was seen. Hence, T helper cell dependence differs between primary and secondary B-cell responses as well as between memory B-cell proliferation and PC differentiation.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute for Immunology (discontinued)
04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Rheumatology, Clinical Immunology and Allergology

UniBE Contributor:

Vogel, Monique and Bachmann, Martin

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0019-2805

Publisher:

Wiley-Blackwell

Language:

English

Submitter:

Eveline Messerli

Date Deposited:

14 Mar 2017 16:55

Last Modified:

06 Jan 2018 02:30

Publisher DOI:

10.1111/imm.12688

PubMed ID:

27861835

Uncontrolled Keywords:

T helper cell-dependent immune response; antibodies; memory B cells; plasma cell differentiation; secondary response

BORIS DOI:

10.7892/boris.94913

URI:

https://boris.unibe.ch/id/eprint/94913

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