Generation of a molecular clone of an attenuated lentivirus, a first step in understanding cytopathogenicity and virulence.

Blatti-Cardinaux, Laure Sarah Pauline; Pisoni, Giuliano; Stoffel, Michael Hubert; Zanoni, Reto Giacomo; Zahno, Marie-Luise; Bertoni, Giuseppe (2016). Generation of a molecular clone of an attenuated lentivirus, a first step in understanding cytopathogenicity and virulence. Virology, 487, pp. 50-58. Academic Press 10.1016/j.virol.2015.09.027

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Small ruminant lentiviruses infect goats and sheep, inducing clinical disease in a minority of infected animals. Following an eradication campaign, clinical cases may disappear in a population. The complete elimination of these lentiviruses is however difficult to achieve and the spreading of less virulent strains often parallels the elimination of their virulent counterparts. Here, we characterized three such strains isolated from a flock in the post-eradication phase. We completely sequenced their genomes, showing that one of the isolates was most probably the product of a recombination event between the other two viruses. By comparing the sequences of these isolates with those of virulent strains, we found evidence that particular LTR mutations may explain their attenuated phenotype. Finally, we constructed an infectious molecular clone representative of these viruses, analyzing its replication characteristics in different target cells. This clone will permit us to explore the molecular correlates of cytopathogenicity and virulence.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Anatomy
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)
09 Interdisciplinary Units > Microscopy Imaging Center MIC

UniBE Contributor:

Blatti, Laure Sarah Pauline; Stoffel, Michael Hubert; Zanoni, Reto Giacomo; Zahno, Marie-Luise and Bertoni, Giuseppe

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0042-6822

Publisher:

Academic Press

Language:

English

Submitter:

Miriam Francine Heinzelmann

Date Deposited:

19 Jul 2017 14:43

Last Modified:

11 Jan 2019 13:44

Publisher DOI:

10.1016/j.virol.2015.09.027

PubMed ID:

26517396

Uncontrolled Keywords:

Caprine arthritis encephalitis virus; Cytopathogenicity; Electron microscopy; Infectious clone; Long terminal repeats; Molecular clone; Small ruminant lentiviruses; Transcription; Virus replication; Visna/maedi virus

BORIS DOI:

10.7892/boris.95021

URI:

https://boris.unibe.ch/id/eprint/95021

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