An in vitro study of fibrin sealant as a carrier system for recombinant human bone morphogenetic protein (rhBMP)–9 for bone tissue engineering

Kobayashi, Masako; Mottini, Matthias; Kobayashi, Eizaburo; Zhang, Yufeng; Schaller, Benoît; Miron, Richard John (2017). An in vitro study of fibrin sealant as a carrier system for recombinant human bone morphogenetic protein (rhBMP)–9 for bone tissue engineering. Journal of cranio-maxillo-facial surgery, 45(1), pp. 27-32. Elsevier 10.1016/j.jcms.2016.10.008

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In the craniofacial bone field, fibrin sealants are used as coagulant and adhesive tools to stabilize grafts during surgery. Despite this, their exact role in osteogenesis is poorly characterized. In the present study, we aimed to characterize the osteogenic potential of TISSEEL fibrin sealant and used its technology to incorporate growth factors within its matrix. We focused on recombinant human bone morphogenetic protein (rhBMP)-9, which has previously been characterized as one of the strongest osteogenetic inducers in the BMP family. TISSEEL displayed an excellent ability to retain rhBMP9, which was gradually released over a 10-day period. Although TISSEEL decreased bone stromal ST2 cell attachment at 8 h, it displayed normal cell proliferation at 1, 3, and 5 days when compared to tissue culture plastic. Interestingly, TISSEEL had little influence on osteoblast differentiation; however its combination with rhBMP9 significantly increased ALP activity at 7 days, Alizarin Red staining at 14 days, and mRNA levels of osteoblast differentiation markers ALP, bone sialoprotein, and osteocalcin. In summary, although fibrin sealants were shown to have little influence on osteogenesis, their combination with bone-inducing growth factors such as rhBMP9 may serve as an attractive carrier/scaffold for future bone regenerative strategies. Future animal studies are now necessary. Copyright © 2016 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved. KEYWORDS: BMP9; Bone morphogenetic proteins; Bone regeneration; Fibrin glue; Fibrin sealant; Osteogenesis

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Craniomaxillofacial Surgery

UniBE Contributor:

Kobayashi, Masako; Mottini, Matthias; Kobayashi, Eizaburo; Schaller, Benoît and Miron, Richard John

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1010-5182

Publisher:

Elsevier

Language:

English

Submitter:

Caroline Dominique Zürcher

Date Deposited:

17 May 2017 14:26

Last Modified:

17 May 2017 14:31

Publisher DOI:

10.1016/j.jcms.2016.10.008

PubMed ID:

27840120

Uncontrolled Keywords:

BMP9; Bone morphogenetic proteins; Bone regeneration; Fibrin glue; Fibrin sealant; Osteogenesis

BORIS DOI:

10.7892/boris.95121

URI:

https://boris.unibe.ch/id/eprint/95121

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