Isolation of yeast complex IV in native lipid nanodiscs

Smirnova, Irina A.; Sjöstrand, Dan; Li, Fei; Björck, Markus; Schäfer, Jacob; Östbye, Henrik; Högbom, Martin; von Ballmoos, Christoph; Lander, Gabriel C.; Ädelroth, Pia; Brzezinski, Peter (2016). Isolation of yeast complex IV in native lipid nanodiscs. Biochimica et biophysica acta - biomembranes, 1858(12), pp. 2984-2992. Elsevier 10.1016/j.bbamem.2016.09.004

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We used the amphipathic styrene maleic acid (SMA) co-polymer to extract cytochrome c oxidase (CytcO) in its native lipid environment from S. cerevisiae mitochondria. Native nanodiscs containing one CytcO per disc were purified using affinity chromatography. The longest cross-sections of the native nanodiscs were 11nm×14nm. Based on this size we estimated that each CytcO was surrounded by ~100 phospholipids. The native nanodiscs contained the same major phospholipids as those found in the mitochondrial inner membrane. Even though CytcO forms a supercomplex with cytochrome bc1 in the mitochondrial membrane, cyt. bc1 was not found in the native nanodiscs. Yet, the loosely-bound Respiratory SuperComplex factors were found to associate with the isolated CytcO. The native nanodiscs displayed an O2-reduction activity of ~130 electrons CytcO-1s-1 and the kinetics of the reaction of the fully reduced CytcO with O2 was essentially the same as that observed with CytcO in mitochondrial membranes. The kinetics of CO-ligand binding to the CytcO catalytic site was similar in the native nanodiscs and the mitochondrial membranes. We also found that excess SMA reversibly inhibited the catalytic activity of the mitochondrial CytcO, presumably by interfering with cyt. c binding. These data point to the importance of removing excess SMA after extraction of the membrane protein. Taken together, our data shows the high potential of using SMA-extracted CytcO for functional and structural studies.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

von Ballmoos, Christoph

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

0005-2736

Publisher:

Elsevier

Language:

English

Submitter:

Christina Schüpbach

Date Deposited:

14 Feb 2017 10:15

Last Modified:

14 Sep 2017 02:30

Publisher DOI:

10.1016/j.bbamem.2016.09.004

BORIS DOI:

10.7892/boris.95324

URI:

https://boris.unibe.ch/id/eprint/95324

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