Receptor FGFRL1 acts as a tumor suppressor in nude mice when overexpressed in HEK 293 Tet-On cells.

Zhuang, Lei; Steinberg, Florian; Trueb, Beat (2016). Receptor FGFRL1 acts as a tumor suppressor in nude mice when overexpressed in HEK 293 Tet-On cells. Oncology letters, 12(6), pp. 4524-4530. Spandidos Publications 10.3892/ol.2016.5245

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Fibroblast growth factor receptor-like 1 (FGFRL1) is a transmembrane receptor that interacts with heparin and FGF ligands. In contrast to the classical FGF receptors, FGFR1 to FGFR4, it does not appear to affect cell growth and proliferation. In the present study, an inducible gene expression system was utilized in combination with a xenograft tumor model to investigate the effects of FGFRL1 on cell adhesion and tumor formation. It was determined that recombinant FGFRL1 promotes the adhesion of HEK 293 Tet-On(®) cells in vitro. Moreover, when such cells are induced to express FGFRL1ΔC they aggregate into huge clusters. If injected into nude mice, the cells form large tumors. Notably, this tumor growth is completely inhibited when the expression of FGFRL1 is induced. The forced expression of FGFRL1 in the tumor tissue may restore contact inhibition, thereby preventing growth of the cells in nude mice. The results of the present study demonstrate that FGFRL1 acts as a tumor suppressor similar to numerous other cell adhesion proteins. It is therefore likely that FGFRL1 functions as a regular cell-cell adhesion protein.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Rheumatology, Clinical Immunology and Allergology > Rheumatology (discontinued)
04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Rheumatology, Clinical Immunology and Allergology

UniBE Contributor:

Zhuang, Lei and Trueb, Beat

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1792-1074

Publisher:

Spandidos Publications

Language:

English

Submitter:

Stefan Kuchen

Date Deposited:

18 Apr 2017 16:09

Last Modified:

18 Apr 2017 16:09

Publisher DOI:

10.3892/ol.2016.5245

PubMed ID:

28101211

Uncontrolled Keywords:

FGF; FGFR; FGFRL1; cell adhesion; tumor suppressor

BORIS DOI:

10.7892/boris.95677

URI:

https://boris.unibe.ch/id/eprint/95677

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