Receptor FGFRL1 does not promote cell proliferation but induces cell adhesion.

Yang, Xiaochen; Steinberg, Florian; Zhuang, Lei; Bessey, Ralph; Trueb, Beat (2016). Receptor FGFRL1 does not promote cell proliferation but induces cell adhesion. International journal of molecular medicine, 38(1), pp. 30-38. Spandidos Publications 10.3892/ijmm.2016.2601

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Fibroblast growth factor receptor (FGFR)-like protein 1 (FGFRL1) is the most recently discovered member of the FGFR family. Owing to the fact that it interacts with FGF ligands, but lacks the intracellular tyrosine kinase domain, several researchers have speculated that it may function as a decoy receptor and exert a negative effect on cell proliferation. In this study, we performed overexpression experiments with TetOn‑inducible cell clones and downregulation experiments with siRNA oligonucleotides, and found that FGFRL1 had absolutely no effect on cell growth and proliferation. Likewise, we did not observe any influence of FGFRL1 on ERK1/2 activation and on the phosphorylation of 250 other signaling proteins analyzed by the Kinexus antibody microarray. On the other hand, with bacterial petri dishes, we observed a clear effect of FGFRL1 on cell adhesion during the initial hours after cell seeding. Our results suggest that FGFRL1 is a cell adhesion protein similar to the nectins rather than a signaling receptor similar to FGFR1-FGFR4.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Rheumatology, Clinical Immunology and Allergology > Rheumatology (discontinued)

UniBE Contributor:

Trueb, Beat

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1107-3756

Publisher:

Spandidos Publications

Language:

English

Submitter:

Stefan Kuchen

Date Deposited:

18 Apr 2017 16:16

Last Modified:

18 Apr 2017 16:16

Publisher DOI:

10.3892/ijmm.2016.2601

PubMed ID:

27220341

BORIS DOI:

10.7892/boris.95678

URI:

https://boris.unibe.ch/id/eprint/95678

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