Acute phase response in lactating dairy cows during hyperinsulinemic hypoglycaemic and hyperinsulinemic euglycaemic clamps and after intramammary LPS challenge.

De Matteis, L; Bertoni, G; Lombardelli, R; Wellnitz, Olga; van Dorland, Hendrika Anette; Vernay, Martin César; Bruckmaier, Rupert; Trevisi, E (2017). Acute phase response in lactating dairy cows during hyperinsulinemic hypoglycaemic and hyperinsulinemic euglycaemic clamps and after intramammary LPS challenge. Journal of animal physiology and animal nutrition, 101(3), pp. 511-520. Blackwell Science 10.1111/jpn.12463

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The link between energy availability, turnover of energy substrates and the onset of inflammation in dairy cows is complex and poorly investigated. To clarify this, plasma inflammatory variables were measured in mid-lactating dairy cows allocated to three groups: hyperinsulinemic hypoglycaemic clamp, induced by insulin infusion (HypoG, n = 5); hyperinsulinemic euglycaemic clamp, induced by insulin and glucose infusion (EuG; n = 6); control, receiving a saline solution infusion (NaCl; n = 6). At 48 h after the start of i.v. infusions, two udder quarters per cow were challenged with 200 μg of E. coli lipopolysaccharide (LPS). Individual blood samples were taken before clamps, before LPS challenge (i.e. 48 h after clamps) and 6.5 h after. At 48 h, positive acute phase proteins (posAPP) did not differ among groups, whereas albumin and cholesterol (index of lipoproteins), negative APP (negAPP), were lower (p < 0.05) in EuG compared to NaCl and HypoG. The concentration of IL-6 was greater in EuG (p < 0.05) but only vs. HypoG. At 6.5 h following LPS challenge, IL-6 increased in the NaCl and EuG clamps (p < 0.05), while TNF-α increased (p < 0.05) in the EuG only. Among the posAPP, haptoglobin markedly increased in EuG (p < 0.05), but not in NaCl (p = 0.76) and in HypoG; ceruloplasmin tended to decline during LPS challenge, the reduction was significant when all animals were considered (p < 0.05). Conversely, all the negAPP showed a marked reduction 6.5 h after LPS challenge in the three groups. In conclusion, EuG caused an inflammatory status after 48-h infusion (i.e. decrease of negAPP) and induced a quicker acute phase response (e.g. marked rise of TNF-α, IL-6) after the intramammary LPS challenge. These data suggest that the simultaneous high availability of glucose and insulin at the tissue-level makes dairy cows more susceptible to inflammatory events. In contrast, HypoG seems to attenuate the inflammatory response.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Physiology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)

UniBE Contributor:

Wellnitz, Olga; van Dorland, Hendrika Anette; Vernay, Martin César and Bruckmaier, Rupert

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

0931-2439

Publisher:

Blackwell Science

Language:

English

Submitter:

Lorenzo Enrique Hernandez Castellano

Date Deposited:

26 Jul 2017 16:42

Last Modified:

26 Jul 2017 16:42

Publisher DOI:

10.1111/jpn.12463

PubMed ID:

27079943

Uncontrolled Keywords:

glucose infusion; inflammation; insulin infusion; interleukin-6; mammary endotoxin challenge; tumour necrosis factor-alpha

BORIS DOI:

10.7892/boris.95857

URI:

https://boris.unibe.ch/id/eprint/95857

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