The Macronuclear Genome of Stentor coeruleus Reveals Tiny Introns in a Giant Cell.

Slabodnick, Mark M; Ruby, J Graham; Reiff, Sarah B; Swart, Estienne Carl; Gosai, Sager; Prabakaran, Sudhakaran; Witkowska, Ewa; Larue, Graham E; Fisher, Susan; Freeman, Robert M; Gunawardena, Jeremy; Chu, William; Stover, Naomi A; Gregory, Brian D; Nowacki, Mariusz; Derisi, Joseph; Roy, Scott William; Marshall, Wallace F; Sood, Pranidhi (2017). The Macronuclear Genome of Stentor coeruleus Reveals Tiny Introns in a Giant Cell. Current Biology, 27(4), pp. 569-575. Cell Press 10.1016/j.cub.2016.12.057

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The giant, single-celled organism Stentor coeruleus has a long history as a model system for studying pattern formation and regeneration in single cells. Stentor [1, 2] is a heterotrichous ciliate distantly related to familiar ciliate models, such as Tetrahymena or Paramecium. The primary distinguishing feature of Stentor is its incredible size: a single cell is 1 mm long. Early developmental biologists, including T.H. Morgan [3], were attracted to the system because of its regenerative abilities-if large portions of a cell are surgically removed, the remnant reorganizes into a normal-looking but smaller cell with correct proportionality [2, 3]. These biologists were also drawn to Stentor because it exhibits a rich repertoire of behaviors, including light avoidance, mechanosensitive contraction, food selection, and even the ability to habituate to touch, a simple form of learning usually seen in higher organisms [4]. While early microsurgical approaches demonstrated a startling array of regenerative and morphogenetic processes in this single-celled organism, Stentor was never developed as a molecular model system. We report the sequencing of the Stentor coeruleus macronuclear genome and reveal key features of the genome. First, we find that Stentor uses the standard genetic code, suggesting that ciliate-specific genetic codes arose after Stentor branched from other ciliates. We also discover that ploidy correlates with Stentor's cell size. Finally, in the Stentor genome, we discover the smallest spliceosomal introns reported for any species. The sequenced genome opens the door to molecular analysis of single-cell regeneration in Stentor.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Ecology and Evolution (IEE) > Evolutionary Ecology
08 Faculty of Science > Department of Biology > Institute of Cell Biology > Ciliate
08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Swart, Estienne Carl; Nowacki, Mariusz and Roy, Scott William

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

0960-9822

Publisher:

Cell Press

Language:

English

Submitter:

Mariusz Nowacki

Date Deposited:

25 Jul 2017 16:30

Last Modified:

25 Jul 2017 16:30

Publisher DOI:

10.1016/j.cub.2016.12.057

PubMed ID:

28190732

Uncontrolled Keywords:

U2, snRNA, cell size, ciliate, genetic code, heterotrichidae, intron, evolution, macronucleus, ploidy, regeneration, splicing

BORIS DOI:

10.7892/boris.95990

URI:

https://boris.unibe.ch/id/eprint/95990

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