Circular Concatemers of Ultra-Short DNA Segments Produce Regulatory RNAs

Allen, Sarah Elizabeth; Hug, Iris Sandra; Pabian, Sylwia Weronika; Rzeszutek, Iwona Maria; Höhener, Cristina; Nowacki, Mariusz (2017). Circular Concatemers of Ultra-Short DNA Segments Produce Regulatory RNAs. Cell, 168(6), 990-999.e7. Cell Press 10.1016/j.cell.2017.02.020

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In the ciliated protozoan Paramecium tetraurelia, Piwi-associated small RNAs are generated upon the elimination of tens of thousands of short transposon-derived DNA segments as part of development. These RNAs then target complementary DNA for elimination in a positive feedback process, contributing to germline defense and genome stability. In this work, we investigate the formation of these RNAs, which we show to be transcribed directly from the short (length mode 27 bp) excised DNA segments. Our data support a mechanism whereby the concatenation and circularization of excised DNA segments provides a template for RNA production. This process allows the generation of a double-stranded RNA for Dicer-like protein cleavage to give rise to a population of small regulatory RNAs that precisely match the excised DNA sequences. VIDEO ABSTRACT.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Allen, Sarah Elizabeth; Hug, Iris Sandra; Pabian, Sylwia Weronika; Rzeszutek, Iwona Maria; Höhener, Cristina and Nowacki, Mariusz

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

0092-8674

Publisher:

Cell Press

Language:

English

Submitter:

Mariusz Nowacki

Date Deposited:

22 Mar 2017 12:00

Last Modified:

22 Mar 2017 12:00

Publisher DOI:

10.1016/j.cell.2017.02.020

PubMed ID:

28283070

Uncontrolled Keywords:

DNA concatemers; DNA elimination; DNA repair; Dicer; Ligase IV; Paramecium; Piwi-interacting RNA; ciliates; circular DNA; small RNA; transcription; transposable elements

BORIS DOI:

10.7892/boris.97052

URI:

https://boris.unibe.ch/id/eprint/97052

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