CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry.

Vig, M; Peinelt, Christine; Beck, A; Koomoa, D L; Rabah, D; Koblan-Huberson, M; Kraft, S; Turner, H; Fleig, A; Penner, R; Kinet, J-P (2006). CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry. Science, 312(5777), pp. 1220-1223. American Association for the Advancement of Science 10.1126/science.1127883

[img] Text
1220.full.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (907kB) | Request a copy

Store-operated Ca2+ entry is mediated by Ca2+ release-activated Ca2+ (CRAC) channels following Ca2+ release from intracellular stores. We performed a genome-wide RNA interference (RNAi) screen in Drosophila cells to identify proteins that inhibit store-operated Ca2+ influx. A secondary patch-clamp screen identified CRACM1 and CRACM2 (CRAC modulators 1 and 2) as modulators of Drosophila CRAC currents. We characterized the human ortholog of CRACM1, a plasma membrane-resident protein encoded by gene FLJ14466. Although overexpression of CRACM1 did not affect CRAC currents, RNAi-mediated knockdown disrupted its activation. CRACM1 could be the CRAC channel itself, a subunit of it, or a component of the CRAC signaling machinery.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine

UniBE Contributor:

Peinelt, Christine

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0036-8075

Publisher:

American Association for the Advancement of Science

Language:

English

Submitter:

Christine Peinelt

Date Deposited:

18 Jun 2018 10:43

Last Modified:

23 Oct 2019 05:20

Publisher DOI:

10.1126/science.1127883

PubMed ID:

16645049

BORIS DOI:

10.7892/boris.97467

URI:

https://boris.unibe.ch/id/eprint/97467

Actions (login required)

Edit item Edit item
Provide Feedback