Kinetics of the Ca(2+), H(+), and Mg(2+) interaction with the ion-binding sites of the SR Ca-ATPase.

Peinelt, Christine; Apell, Hans-Jürgen (2002). Kinetics of the Ca(2+), H(+), and Mg(2+) interaction with the ion-binding sites of the SR Ca-ATPase. Biophysical journal, 82(1 Pt 1), pp. 170-181. Biophysical Society 10.1016/S0006-3495(02)75384-8

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Electrochromic styryl dyes were used to investigate mutually antagonistic effects of Ca(2+) and H(+) on binding of the other ion in the E(1) and P-E(2) states of the SR Ca-ATPase. On the cytoplasmic side of the protein in the absence of Mg(2+) a strictly competitive binding sequence, H(2)E(1) <==> HE(1) <==> E(1) <==> CaE(1) <==> Ca(2)E(1), was found with two Ca(2+) ions bound cooperatively. The apparent equilibrium dissociation constants were in the order of K(1/2)(2 Ca) = 34 nM, K(1/2)(H) = 1 nM and K(1/2)(H(2)) = 1.32 microM. Up to 2 Mg(2+) ions were also able to enter the binding sites electrogenically and to compete with the transported substrate ions (K(1/2)(Mg) = 165 microM, K(1/2)(Mg(2)) = 7.4 mM). In the P-E(2) state, with binding sites facing the lumen of the sarcoplasmatic reticulum, the measured concentration dependence of Ca(2+) and H(+) binding could be described satisfactorily only with a branched reaction scheme in which a mixed state, P-E(2)CaH, exists. From numerical simulations, equilibrium dissociation constants could be determined for Ca(2+) (0.4 mM and 25 mM) and H(+) (2 microM and 10 microM). These simulations reproduced all observed antagonistic concentration dependences. The comparison of the dielectric ion binding in the E(1) and P-E(2) conformations indicates that the transition between both conformations is accompanied by a shift of their (dielectric) position.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine

UniBE Contributor:

Peinelt, Christine

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0006-3495

Publisher:

Biophysical Society

Language:

English

Submitter:

Christine Peinelt

Date Deposited:

14 Jun 2018 16:02

Last Modified:

14 Jun 2018 16:02

Publisher DOI:

10.1016/S0006-3495(02)75384-8

PubMed ID:

11751306

BORIS DOI:

10.7892/boris.97471

URI:

https://boris.unibe.ch/id/eprint/97471

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