Steinbach, Christoph; Kroupová, Hana Kocour; Wahli, Thomas; Klicnarová, Jana; Schmidt-Posthaus, Heike (2016). Histopathological alterations of the heart in fish: proposal for a standardized assessment. Diseases of aquatic organisms, 118(3), pp. 185-194. Inter-Research 10.3354/dao02971
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Histopathological alterations in the heart are often reported in fish as a result of exposure to a variety of chemical compounds. However, researchers presently lack a standardized method for the evaluation of histopathological alterations in the cardiovascular system of fish and the calculation of an 'organ index'. Therefore, we designed a method for a standardized assessment and evaluation of histopathological alterations in the heart of fish. As a model species, we used rainbow trout Oncorhynchus mykiss, but the protocol was also successfully applied to other fish species belonging to different taxonomic orders. To test the protocol, we re-evaluated sections of atenolol-exposed and unexposed rainbow trout obtained in a previous study. The results were in accordance with those previously published, demonstrating the applicability of the protocol. The protocol provides a universal method for the comparative evaluation of histopathological changes in the heart of fish.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute for Fish and Wildlife Health (FIWI) |
UniBE Contributor: |
Steinbach, Christoph, Wahli, Thomas, Schmidt-Posthaus, Heike |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
0177-5103 |
Publisher: |
Inter-Research |
Language: |
English |
Submitter: |
Lucia Gugger-Raaflaub |
Date Deposited: |
21 Jul 2017 13:19 |
Last Modified: |
05 Dec 2022 15:04 |
Publisher DOI: |
10.3354/dao02971 |
PubMed ID: |
27025306 |
BORIS DOI: |
10.7892/boris.97754 |
URI: |
https://boris.unibe.ch/id/eprint/97754 |
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