Phosphorylation of tobacco eukaryotic translation initiation factor 4A upon pollen tube germination

op den Camp, Roel G. L.; Kuhlemeier, Cris (1998). Phosphorylation of tobacco eukaryotic translation initiation factor 4A upon pollen tube germination. Nucleic acids research, 26(9), pp. 2058-2062. Oxford University Press 10.1093/nar/26.9.2058

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Eukaryotic translation initiation factor eIF-4A is a member of the DEAD box family of RNA helicases and RNA-dependent ATPases, In tobacco, eIF-4A is encoded by a gene family with one isoform, eIF-4A8, being exclusively expressed in pollen. This pollen-specific isoform is a candidate for mediating translational control in the developing gametophyte. Here we show that eIF-4A is barely phosphorylated in mature pollen, but during pollen tube germination, two isoforms of eIF-4A become phosphorylated. Phosphoamino acid analysis indicated phosphorylation of threonine. In order to determine whether pollen-specific eIF-4A8 is among the phosphorylated isoforms, we raised transgenic tobacco plants overexpressing eIF-4A8 containing a histidine tag. Hereby, we could show that indeed eIF-4A8 is modified through phosphorylation. The biological relevance of the phosphorylation of eIF-4A is discussed.

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