Aldehyde dehydrogenase in tobacco pollen

op den Camp, Roel GL; Kuhlemeier, Cris (1997). Aldehyde dehydrogenase in tobacco pollen. Plant molecular biology, 35(3), pp. 355-365. Kluwer Academic Publishers 10.1023/A:1005889129491

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Acetaldehyde is one of the intermediate products of ethanolic fermentation, which can be reduced to ethanol by alcohol dehydrogenase (ADH). Alternatively, acetaldehyde can be oxidized to acetate by aldehyde dehydrogenase (ALDH) and subsequently converted to acetyl-CoA by acetyl-CoA synthetase (ACS). To study the expression of ALDHs in plants we isolated and characterized a cDNA coding for a putative mitochondrial ALDH (TobAldh2A) in Nicotiana tabacum. TobALDH2A shows 54-60% identity at the amino acid level with other ALDHs and shows 76% identity with maize Rf2, a gene involved in restoration of male fertility in cms-T maize. TobAldh2A transcripts and protein were present at high levels in the male and female reproductive tissues. Expression in vegetative tissues was much lower and no induction by anaerobic incubation was observed. This suggests that TobALDH expression is not part of the anaerobic response, but may have another function. The use of specific inhibitors of ALDH and the pyruvate dehydrogenase (PDH) complex indicates that ALDH activity is important for pollen tube growth, and thus may have a function in biosynthesis or energy production.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development

UniBE Contributor:

Kuhlemeier, Cris

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0167-4412

Publisher:

Kluwer Academic Publishers

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

06 Jul 2017 14:15

Last Modified:

09 Nov 2019 19:16

Publisher DOI:

10.1023/A:1005889129491

Uncontrolled Keywords:

aldehyde dehydrogenase, cytoplasmic male sterility, fermentation, glyoxylate cycle, pollen, tobacco

BORIS DOI:

10.7892/boris.97858

URI:

https://boris.unibe.ch/id/eprint/97858

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