AEROBIC FERMENTATION IN TOBACCO POLLEN

Bucher, Marcel; Brander, Karl; Sbicego, Sandro; Mandel, Therese; Kuhlemeier, Cris (1995). AEROBIC FERMENTATION IN TOBACCO POLLEN. Plant molecular biology, 28(4), pp. 739-750. Springer 10.1007/BF00021197

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We characterized the genes coding for the two dedicated enzymes of ethanolic fermentation, alcohol dehydrogenase (ADH) and pyruvate decarboxylase (PDC), and show that they are functional in pollen. Two PDC-encoding genes were isolated, which displayed reciprocal regulation: PDC1 was anaerobically induced in leaves, whereas PDC2 mRNA was absent in leaves, but constitutively present in pollen. A flux through the ethanolic fermentation pathway could be measured in pollen under all tested environmental and developmental conditions. Surprisingly, the major factor influencing the rate of ethanol production was not oxygen availability, but the composition of the incubation medium. Under optimal conditions for pollen tube growth, approximately two-thirds of the carbon consumed was fermented, and ethanol accumulated into the surrounding medium to a concentration exceeding 100 mM.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Mandel, Therese and Kuhlemeier, Cris

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0167-4412

Publisher:

Springer

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

05 Jul 2017 10:27

Last Modified:

05 Jul 2017 10:27

Publisher DOI:

10.1007/BF00021197

Uncontrolled Keywords:

alcohol dehydrogenase, fermentation, gene expression, pollen, pyruvate decarboxylase, respiration, tobacco

BORIS DOI:

10.7892/boris.97864

URI:

https://boris.unibe.ch/id/eprint/97864

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