Long-Term Anoxia Tolerance (Multi-Level Regulation of Gene Expression in the Amphibious Plant Acorus calamus L.)

Bucher, Marcel; Kuhlemeier, Cris (1993). Long-Term Anoxia Tolerance (Multi-Level Regulation of Gene Expression in the Amphibious Plant Acorus calamus L.). Plant Physiology, 103(2), pp. 441-448. American Society of Plant Physiologists 10.1104/pp.103.2.441

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Acorus calamus is a monocotyledonous wetland plant that can withstand extremely long periods of anoxia. We have investigated the expression of genes coding for pyruvate decarboxylase (Pdc), alcohol dehydrogenase (Adh), and fructose-1,6-bisphosphate aldolase (Ald) during periods of anoxia ranging from 2 h to 2 months. Upon anoxic incubation, Pdc mRNA levels peak at 6 h, followed by Adh and Ald, which peak at 12 and 72 h, respectively. Subsequently, the mRNA levels of all three genes decline within days to low levels. In contrast, alcohol dehydrogenase (ADH) protein levels increase steadily for at least a week and then remain constant. Native gel electrophoresis demonstrates the presence of two sets of ADH isozymes, one present constitutively, the other enhanced during anoxia. Translation initiation factor 4A protein levels, used as a control, remain constant during 2 months of anoxia. The results suggest that A. calamus has developed a complex anaerobic response consisting of differential regulation of transcription, translation, and posttranslational processes.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Kuhlemeier, Cris

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0032-0889

Publisher:

American Society of Plant Physiologists

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

05 Jul 2017 08:44

Last Modified:

05 Jul 2017 08:44

Publisher DOI:

10.1104/pp.103.2.441

BORIS DOI:

10.7892/boris.97870

URI:

https://boris.unibe.ch/id/eprint/97870

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