Differential expression of genes encoding the hypusine-containing translation initiation factor, elF-5A, in tobacco

Chamot, Danuta; Kuhlemeier, Cris (1992). Differential expression of genes encoding the hypusine-containing translation initiation factor, elF-5A, in tobacco. Nucleic acids research, 20(4), pp. 665-669. Information Retrieval Ltd. 10.1093/nar/20.4.665

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Two Nicotiana plumbaginifolia cDNA clones, NeIF-5A1 and NeIF-5A2, encoding eukaryotic translation initiation factor eIF-5A (formerly called eIF-4D) were cloned by heterologous screening with Dictyostelium and human eIF-5A probes. eIF-5A is the only protein known to contain a unique amino acid modification, hypusine. Comparison of the Nicotiana deduced amino acid sequences with those of other eIF-5A polypeptides reveals conservation throughout the coding sequence, especially in the region of the hypusine residue. Transcript analysis reveals that NeIF-5A1 is preferentially expressed in photosynthetic tissues, while NeIF-5A2 is constitutively expressed in all plant tissues examined. A polyclonal antibody was raised against NeIF-5A1 overexpressed in E. coli. NeIF-5A1 antiserum crossreacts with an 18 kDa polypeptide doublet in all tobacco tissues examined. At least one polypeptide of ca. 18 kDa from a diversity of higher and lower plants crossreacts with NeIF-5A1 antiserum.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Kuhlemeier, Cris

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0305-1048

Publisher:

Information Retrieval Ltd.

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

04 Jul 2017 10:47

Last Modified:

04 Jul 2017 10:47

Publisher DOI:

10.1093/nar/20.4.665

Uncontrolled Keywords:

amino acid sequence; clone cells; DNA, complementary; genes; immune sera; open reading frames; peptide initiation factors; plants tobacco; polyclonal antibody

BORIS DOI:

10.7892/boris.97874

URI:

https://boris.unibe.ch/id/eprint/97874

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