Divergent genes for translation inition factor elF-4A are coordinately expressed in tobacco

Owttrim, George W; Hofmann, Sylvia; Kuhlemeier, Cris (1991). Divergent genes for translation inition factor elF-4A are coordinately expressed in tobacco. Nucleic acids research, 19(20), pp. 5491-5496. Information Retrieval Ltd. 10.1093/nar/19.20.5491

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Three CDNA clones coding for eukaryotic translation initiation factor 4A, elF-4A, were isolated from a Nicotiana plumbaginifolia root cDNA library by heterologous screening. The clones comprise two distinct gene classes as two clones are highly similar while the third is divergent. The genes belong to a highly conserved gene family, the DEAD box supergene family, although the divergent clone contains a DESD box rather than the characteristic DEAD box. The two clones are representatives of separate small multigene families in both N. plumbaginifolia and N. tabacum. Representatives of each family are coordinately expressed in all plant organs examined. The 47 kD polypeptide product of one clone, overexpressed in E. coli, crossreacts immunologically with a rabbit reticulocyte elF-4A polyclonal antibody. Taken together the data suggest that the two Nicotiana elF-4A genes encode translation initiation factors. The sequence divergence and the coordinate expression of the two Nicotiana elF-4A families provide an excellent system to determine if functionally distinct elF-4A polypeptides are required for translation initiation in plants.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Kuhlemeier, Cris

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0305-1048

Publisher:

Information Retrieval Ltd.

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

04 Jul 2017 10:42

Last Modified:

04 Jul 2017 10:42

Publisher DOI:

10.1093/nar/19.20.5491

Uncontrolled Keywords:

clone cells; DNA, complementary; genes; libraries; peptide initiation factors; peptides; plants; oryctolagus cuniculus; reticulocytes; tobacco; kawasaki's disease; polyclonal antibody; ketogenic diet

BORIS DOI:

10.7892/boris.97875

URI:

https://boris.unibe.ch/id/eprint/97875

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