The patterns of gene expression in the tomato shoot apical meristem.

Fleming, Andrew J; Mandel, Therese; Roth, I; Kuhlemeier, Cris (1993). The patterns of gene expression in the tomato shoot apical meristem. The Plant Cell, 5(3), pp. 297-309. American Society of Plant Biologists 10.1105/tpc.5.3.297

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In this paper, we describe the synthesis of a cDNA library from the vegetative shoot apical meristem and the analysis of clones selected from it. Using in situ hybridization, we characterized the patterns of expression of these genes in the tomato shoot apical meristem, as well as the patterns obtained from other sources. The results from the analysis of 15 cDNAs indicated the following six main patterns of gene expression in the shoot apical meristem: overall expression, zero expression, expression limited to the epidermis, expression excluded from the epidermis, punctate expression, and expression elevated in the flanks of the meristem. The patterns observed and the nature and number of the genes showing these patterns necessitate a reinterpretation of the models of meristem structure and function. In particular, the data suggest a compartmentation within the shoot apical meristem based on the spatial expression of particular subsets of genes. This paper also reports on the specific and precise criteria essential for the correct identification of meristem-specific gene expression. The data give new insight into the molecular organization of the shoot apical meristem and provide the framework for a detailed dissection of the factors controlling this organization.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Mandel, Therese and Kuhlemeier, Cris

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

1040-4651

Publisher:

American Society of Plant Biologists

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

04 Jul 2017 11:02

Last Modified:

04 Jul 2017 11:02

Publisher DOI:

10.1105/tpc.5.3.297

PubMed ID:

8467223

BORIS DOI:

10.7892/boris.98811

URI:

https://boris.unibe.ch/id/eprint/98811

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