Plasmids Carrying blaCMY -2/4 in Escherichia coli from Poultry, Poultry Meat, and Humans Belong to a Novel IncK Subgroup Designated IncK2.

Seiffert, Salome Nadja; Carattoli, Alessandra; Schwendener, Sybille; Collaud, Alexandra; Endimiani, Andrea; Perreten, Vincent (2017). Plasmids Carrying blaCMY -2/4 in Escherichia coli from Poultry, Poultry Meat, and Humans Belong to a Novel IncK Subgroup Designated IncK2. Frontiers in Microbiology, 8(407), p. 407. Frontiers 10.3389/fmicb.2017.00407

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The blaCMY -2/4-carrying IncB/O/K-like plasmids of seven Escherichia coli strains from poultry, poultry meat and human urine samples were examined using comparative analysis of whole plasmid sequences. The incompatibility group was determined by analysis of the incRNAI region and conjugation assays with strains containing the IncK and IncB/O reference plasmids. Strains were additionally characterized using MLST and MIC determination. The complete DNA sequences of all plasmids showed an average nucleotide identity of 91.3%. Plasmids were detected in E. coli sequence type (ST) 131, ST38, ST420, ST1431, ST1564 and belonged to a new plasmid variant (IncK2) within the IncK and IncB/O groups. Notably, one E. coli from poultry meat and one from human contained the same plasmid. The presence of a common recently recognized IncK2 plasmid in diverse E. coli from human urine isolates and poultry meat production suggests that the IncK2 plasmids originated from a common progenitor and have the capability to spread to genetically diverse E. coli in different reservoirs. This discovery is alarming and stresses the need of rapidly introducing strict hygiene measures throughout the food chain, limiting the spread of such plasmids in the human settings.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology > Molecular Bacterial Epidemiology and Infectiology
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Seiffert, Salome Nadja; Carattoli, Alessandra; Schwendener, Sybille; Collaud, Alexandra; Endimiani, Andrea and Perreten, Vincent

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1664-302X

Publisher:

Frontiers

Language:

English

Submitter:

Vincent Perreten

Date Deposited:

09 Aug 2017 16:31

Last Modified:

13 Aug 2017 02:13

Publisher DOI:

10.3389/fmicb.2017.00407

PubMed ID:

28360894

Uncontrolled Keywords:

ESBL; animals; antibiotic resistance; food; pAmpC

BORIS DOI:

10.7892/boris.99001

URI:

https://boris.unibe.ch/id/eprint/99001

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