Increased Maternal and Fetal Cholesterol Efflux Capacity and Placental CYP27A1 Expression in Pre-eclampsia.

Mistry, Hiten Dhirubhai; Kurlak, Lesia O; Mansour, Yosef T; Zurkinden, Line; Mohaupt, Markus; Escher, Geneviève (2017). Increased Maternal and Fetal Cholesterol Efflux Capacity and Placental CYP27A1 Expression in Pre-eclampsia. Journal of lipid research, 58(6), pp. 1186-1195. American Society for Biochemistry and Molecular Biology ASBMB 10.1194/jlr.M071985

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Pre-eclampsia is a pregnancy-specific condition, leading to increased cardiovascular risk in later life. A decrease in cholesterol efflux capacity is linked to cardiovascular disease. We hypothesised that, in pre-eclampsia there would be a disruption of maternal/fetal plasma to efflux cholesterol as well as differences in the concentrations of both placental sterol 27-hydroxylase (CYP27A1) and apoA1-binding-protein (AIBP). Total, HDL- and ABCA1-mediated cholesterol efflux were performed with maternal and fetal plasma from women with pre-eclampsia and normotensive controls (both n=17). ApoA1/apoE were quantified by chemiluminescence; 27-hydroxycholesterol (27-OHC) by GC-MS. Immunohistochemistry was used to determine placental expression/localisation of CYP27A1, AIBP, apoA1, apoE and SRB1. Maternal and fetal total and HDL-mediated cholesterol efflux capacities were increased in pre-eclampsia (by 10-20%), but ABCA1-mediated efflux was decreased (by 20-35%; P<0.05). Maternal and fetal apoE concentrations were higher in pre-eclampsia. Fetal plasma 27-OHC levels were decreased in pre-eclamptic samples (P<0.05). Placental protein expression of both CYP27A1 and AIBP were localised around fetal vessels and significantly increased in pre-eclampsia (P=0.04). Placental 27-OHC concentrations were also raised in pre-eclampsia (P<0.05). Increased HDL-mediated cholesterol efflux capacity and placental CYP27A1/27-OHC could be a rescue mechanism in pre-eclampsia, to remove cholesterol from cells to limit lipid peroxidation and increase placental angiogenesis.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Forschungsgruppe Nephrologie / Hypertonie
04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Nephrology and Hypertension

UniBE Contributor:

Mistry, Hiten Dhirubhai; Zurkinden, Line; Mohaupt, Markus and Escher, Geneviève

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0022-2275

Publisher:

American Society for Biochemistry and Molecular Biology ASBMB

Language:

English

Submitter:

Geneviève Escher

Date Deposited:

08 Nov 2017 08:19

Last Modified:

01 Jul 2018 02:32

Publisher DOI:

10.1194/jlr.M071985

PubMed ID:

28396342

Uncontrolled Keywords:

Apolipoproteins Cholesterol Cholesterol/Efflux Hypertension Lipids Oxysterols Placenta Pregnancy

BORIS DOI:

10.7892/boris.99323

URI:

https://boris.unibe.ch/id/eprint/99323

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