Screening of ethyl methane sulphonate mutagenized tef [ Eragrostis tef (Zucc.) Trotter] population identifies Al-tolerant lines

Desta, Ermias A.; Hussein, Shimelis; Laing, Mark; Mengistu, Fentahun; Tadele, Zerihun (2017). Screening of ethyl methane sulphonate mutagenized tef [ Eragrostis tef (Zucc.) Trotter] population identifies Al-tolerant lines. Journal of plant interactions, 12(1), pp. 170-176. Taylor & Francis 10.1080/17429145.2017.1310943

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About 15,000 M2 seeds of ethyl-methane-sulphonate (EMS)-mutagenized population were screened along with Al-tolerant and sensitive checks and the M0 variety. Strongly acidic soil with an external application of a toxic Al-solution and exposure to moisture stress was used to maximize selection pressure. Twenty-one M2 plants with root lengths of greater than the mean of the tolerant check were selected and planted for seed production. Candidate M3 plants were investigated for Al-tolerance and for morpho-agronomic traits under greenhouse and field conditions, respectively. Highly significant differences were observed for Al-tolerance between the candidate mutant lines and the M0 (P < .001), and between mutant lines and the sensitive check (P < .001). Similarly, significant differences were observed between the mutant lines for 16 of the 20 quantitative traits measured. This study is the first to report successful induction of enhanced Al-tolerance in tef by using EMS mutagenized population.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Tadele, Zerihun

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

1742-9145

Publisher:

Taylor & Francis

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

06 Jul 2017 14:55

Last Modified:

06 Jul 2017 14:58

Publisher DOI:

10.1080/17429145.2017.1310943

Uncontrolled Keywords:

aluminium, ethyl methane sulphonate (EMS), mutation breeding, tef

BORIS DOI:

10.7892/boris.99367

URI:

https://boris.unibe.ch/id/eprint/99367

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