Prevalence and distribution of the insertion element ISMag1 in Mycoplasma agalactiae.

Pilo, Paola; Fleury, Bénédicte; Marenda, Marc; Frey, Joachim; Vilei, Edy (2003). Prevalence and distribution of the insertion element ISMag1 in Mycoplasma agalactiae. Veterinary microbiology, 92(1-2), pp. 37-48. Elsevier 10.1016/S0378-1135(02)00311-5

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In characterising Mycoplasma agalactiae strains from various European countries and from Africa, a new insertion sequence (IS), ISMag1, which is related to IS of the family of IS30 insertion elements, has been identified by DNA sequence analysis and Southern blot hybridisation. ISMag1 has a size of 1515bp, and contains inverted repeats of 3bp and a gene encoding the putative transposase on a single open reading frame. ISMag1 is present only in the rarely isolated serotypes E, F, G and H of M. agalactiae, where it is found in 1 to approximately 30 copies. The different patterns obtained by hybridisation of a labelled probe of ISMag1 to genomic DNA cut with various restriction enzymes correlate to some extent to the different serotypes and to variations of the nucleotide sequences of the uvrC genes of the different strains. Based on uvrC sequences, the strains of M. agalactiae carrying ISMag1 form a cluster, separate from the other strains. IS patterns obtained with ISMag1 allow a fine subtyping of the serotypes E, F, G and H of M. agalactiae for epidemiological studies. The potential role of ISMag1 and of its copy numbers on virulence and persistence of the respective strains requests further studies.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Pilo, Paola; Frey, Joachim and Vilei, Edy

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

0378-1135

Publisher:

Elsevier

Language:

English

Submitter:

Paola Pilo

Date Deposited:

14 Jun 2018 10:19

Last Modified:

14 Jun 2018 10:19

Publisher DOI:

10.1016/S0378-1135(02)00311-5

PubMed ID:

12488069

BORIS DOI:

10.7892/boris.99621

URI:

https://boris.unibe.ch/id/eprint/99621

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