Skorczyk, Anna; Stachowiak, Monika; Szczerbal, Izabela; Klukowska-Roetzler, Jolanta; Schelling, Claude; Dolf, Gaudenz; Switonski, Marek (2007). Polymorphism and chromosomal location of the MC4R (melanocortin-4 receptor) gene in the dog and red fox. Gene, 392(1-2), pp. 247-52. Amsterdam: Elsevier 10.1016/j.gene.2006.12.027
Full text not available from this repository. (Request a copy)The melanocortin-4 receptor (MC4R) is expressed in the hypothalamus and regulates energy intake and body weight. In silico screening of the canine chromosome 1 sequence and a comparison with the porcine MC4R sequence by BLAST were performed. The nucleotide sequence of the whole coding region and 3'- and 5'-flanking regions of the dog (1214 bp) and red fox (1177 bp) MC4R gene was established and high conservation of the nucleotide sequences was revealed (99%). Five sets of PCR primers were designed and a search for polymorphism was performed by the SSCP technique in a group of 31 dogs representing nineteen breeds and 35 farm red foxes. Sequencing of DNA fragments, representing the identified SSCP patterns, revealed three single nucleotide polymorphisms (including a missense one) in dogs and four silent SNPs in red foxes. An average SNP frequency was approx. 1/400 bp in the dog and 1/300 bp in the red fox. We mapped the MC4R gene by FISH to the canine chromosome 1 (CFA1q1.1) and to the red fox chromosome 5 (VVU5p1.2).
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Institute of Genetics |
UniBE Contributor: |
Klukowska, Jolanta, Dolf, Gaudenz |
ISSN: |
0378-1119 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:53 |
Last Modified: |
05 Dec 2022 14:16 |
Publisher DOI: |
10.1016/j.gene.2006.12.027 |
PubMed ID: |
17306938 |
Web of Science ID: |
000246078300028 |
URI: |
https://boris.unibe.ch/id/eprint/22716 (FactScience: 36213) |
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