Guex, Anne Géraldine; Fortunato, Giuseppino; Hegemann, Dirk; Tevaearai, Hendrik T; Giraud, Marie-Noëlle (2013). General Protocol for the Culture of Cells on Plasma-Coated Electrospun Scaffolds. In: Stem Cell Nanotechnology 1058 (pp. 119-131). Totowa, N.J.: Humana Press 10.1007/7651_2013_8
Full text not available from this repository. (Request a copy)As opposed to culture on standard tissue-treated plastic, cell culture on three-dimensional scaffolds impedes additional challenges with respect to substrate preparation, cell seeding, culture maintenance, and analysis. We herewith present a general route for the culture of primary cells, differentiated cells, or stem cells on plasma-coated, electrospun scaffolds. We describe a method to prepare and fix the scaffolds in culture wells and discuss a convenient method for cell seeding and subsequent analysis by scanning electron microscopy or immunohistology.
Item Type: |
Book Section (Book Chapter) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Herz- und Gefässchirurgie 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Herz- und Gefässchirurgie 04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Heart Surgery |
UniBE Contributor: |
Guex, Anne Géraldine, Tevaearai, Hendrik |
ISSN: |
1064-3745 |
ISBN: |
978-1-62703-570-5 |
Publisher: |
Humana Press |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:39 |
Last Modified: |
27 Feb 2024 14:29 |
Publisher DOI: |
10.1007/7651_2013_8 |
PubMed ID: |
23700279 |
URI: |
https://boris.unibe.ch/id/eprint/15704 (FactScience: 223133) |
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