General Protocol for the Culture of Cells on Plasma-Coated Electrospun Scaffolds

Guex, Anne Géraldine; Fortunato, Giuseppino; Hegemann, Dirk; Tevaearai, Hendrik T; Giraud, Marie-Noëlle (2013). General Protocol for the Culture of Cells on Plasma-Coated Electrospun Scaffolds. In: Stem Cell Nanotechnology 1058 (pp. 119-131). Totowa, N.J.: Humana Press 10.1007/7651_2013_8

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As opposed to culture on standard tissue-treated plastic, cell culture on three-dimensional scaffolds impedes additional challenges with respect to substrate preparation, cell seeding, culture maintenance, and analysis. We herewith present a general route for the culture of primary cells, differentiated cells, or stem cells on plasma-coated, electrospun scaffolds. We describe a method to prepare and fix the scaffolds in culture wells and discuss a convenient method for cell seeding and subsequent analysis by scanning electron microscopy or immunohistology.

Item Type:	Book Section (Book Chapter)
Division/Institute:	04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Herz- und Gefässchirurgie 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Herz- und Gefässchirurgie 04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Heart Surgery
UniBE Contributor:	Guex, Anne Géraldine, Tevaearai, Hendrik
ISSN:	1064-3745
ISBN:	978-1-62703-570-5
Publisher:	Humana Press
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:39
Last Modified:	27 Feb 2024 14:29
Publisher DOI:	10.1007/7651_2013_8
PubMed ID:	23700279
URI:	https://boris.unibe.ch/id/eprint/15704 (FactScience: 223133)